Rapid and Sensitive Detection of Lymphocystis Disease Virus Genotype VII by Loop-Mediated Isothermal Amplification

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• 作者：Estefanía J. Valverde ; Irene Cano ; Dolores Castro…
• 关键词：Real ; time loop ; mediated isothermal amplification ; Lymphocystis disease virus ; Gilthead seabream ; Senegalese sole ; Carrier fish
• 刊名：Food and Environmental Virology
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：9
• 期：1
• 页码：114-122
• 全文大小：
• 刊物主题：Virology; Food Science; Chemistry/Food Science, general;
• 出版者：Springer US
• ISSN：1867-0342
• 卷排序：9

文摘

Lymphocystis disease virus (LCDV) infections have been described in gilthead seabream (Sparus aurata L.) and Senegalese sole (Solea senegalensis, Kaup), two of the most important marine fish species in the Mediterranean aquaculture. In this study, a rapid, specific, and sensitive detection method for LCDV genotype VII based on loop-mediated isothermal amplification (LAMP) was developed. The LAMP assay, performed using an apparatus with real-time amplification monitoring, was able to specifically detect LCDV genotype VII from clinically positive samples in less than 12 min. In addition, the assay allowed the detection of LCDV in all asymptomatic carrier fish analysed, identified by qPCR, showing an analytical sensitivity of ten copies of viral DNA per reaction. The LCDV LAMP assay has proven to be a promising diagnostic method that can be used easily in fish farms to detect the presence and spread of this iridovirus.