One-step nucleic acid amplification (OSNA): where do we go with it?

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• 作者：Yasuhiro Tamaki
• 关键词：One ; step nucleic acid amplification (OSNA) ; Cytokeratin 19 ; Sentinel lymph node metastasis
• 刊名：International Journal of Clinical Oncology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：22
• 期：1
• 页码：3-10
• 全文大小：
• 刊物类别：Medicine
• 刊物主题：Oncology; Surgical Oncology; Cancer Research;
• 出版者：Springer Japan
• ISSN：1437-7772
• 卷排序：22

文摘

The one-step nucleic acid amplification (OSNA) assay was initially developed for the intraoperative assessment of sentinel lymph node metastases in breast cancer. This assay measures cytokeratin 19 (CK19) mRNA copy number and is widely used in hospitals. The results of the IBCSG 23-01, ACOSOG Z0011, and AMAROS trials demonstrated that no further axillary dissection is required for patients with sentinel lymph nodes that tested positive for cancer, which has led to a decreasing trend in the need for intraoperative assessment of lymph nodes. Here, I review studies relevant to OSNA and discuss perspectives on future applications of OSNA in cancer surgery. The studies reviewed were identified by carrying out a search on PubMed for all articles pertaining to OSNA and published prior to the end of June 2016 using the keywords “OSNA” or “one-step nucleic acid amplification” in the title or abstract. Method comparison studies between OSNA and pathological assessment for the detection of lymph node metastasis in breast cancer revealed that in a pooled assessment OSNA had a high specificity (94.8 %), high concordant rate (93.8 %), and a negative predictive value (97.6 %). Similar results have been found for gastric, colorectal, and lung cancers in multicenter studies. These results demonstrate that OSNA can serve as an alternative method to pathological assessment for examining lymph node metastasis. Multicenter prospective studies with a large sample size are needed to definitively reveal the superiority of OSNA over pathological assessment to predict prognosis. Technical refinements to improve the assay are essential to its further development as a new standard for testing in place of pathological examination.