Rhizobacterium-mediated growth promotion and expression of stress enzymes in Glycine max L. Merrill against Fusarium wilt upon challenge inoculation

详细信息    查看全文

• 作者：Shekhar Jain (1)
  Anukool Vaishnav (1)
  Amrita Kasotia (1)
  Sarita Kumari (1)
  Rajarshi Kumar Gaur (1)
  Devendra Kumar Choudhary (1)
  
• 关键词：Induced systemic resistance ; Fusarium wilt ; Plant growth ; promoting bacteria ; Plant stress enzymes
• 刊名：World Journal of Microbiology and Biotechnology
• 出版年：2014
• 出版时间：February 2014
• 年：2014
• 卷：30
• 期：2
• 页码：399-406
• 全文大小：328 KB
• 作者单位：Shekhar Jain (1)
  Anukool Vaishnav (1)
  Amrita Kasotia (1)
  Sarita Kumari (1)
  Rajarshi Kumar Gaur (1)
  Devendra Kumar Choudhary (1)
  
  1. Department of Science, Faculty of Arts, Science and Commerce (FASC), Mody Institute of Technology and Science (MITS), Lakshmangarh, Sikar, 332311, Rajasthan, India
  
• ISSN：1573-0972

文摘

Wilt disease of soybean caused by a very common soil-borne fungus, Fusarium oxysporum is one of the most destructive diseases of the crop. The aim of the present study was to characterize plant growth-promotion activities and induced resistance of a rhizobacterial strain for the soybean plant against F. oxysporum. Rhizobacterium strain SJ-5 exhibited plant growth-promotion characteristics and antagonistic activity against the test pathogen on dual plate assay. It was identified as a Carnobacterium sp. A 950?bp PCR product was amplified from Carnobacterium sp. strain SJ-5, using zwittermicin A self-resistance gene-specific primers (zmaR). The strain produced indole 3-acetic acid (19?μg/ml) in the presence of salt stress and exhibited growth in Dworkin and Foster salt medium amended with 1-aminocyclopropane-1-carboxylate (ACC) through ACC deaminase activity (277?nmol/mg/h) as compared to the control. Strain seeds treated with the strain significantly enhanced the quorum of healthy plants after challenge inoculation at 14?days after seeding. An increase in the activity of stress enzymes after challenge inoculation with the test pathogen is reported. Treatment with the bacterium resulted in an increase in the chlorophyll content in the leaves in comparison with challenge-inoculated plants.