Purification and identification of α 2- linked sialoglycoprotein and α 2- linked sialoglycoprotein in edible bird’s nest

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• 作者：Yanyan You ; Yan Cao ; Shuang Guo ; Jie Xu…
• 关键词：Edible bird’s nest (EBN) ; Sialoglycoprotein ; Maackia amurensis agglutinin (MAA) ; Sambucus nigra agglutinin (SNA) ; Peptide mapping
• 刊名：European Food Research and Technology
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：240
• 期：2
• 页码：389-397
• 全文大小：862 KB
• 参考文献：1. Wu YJ, Chen Y, Wang B, Bai LQ, Han WR, Ge YQ, Yuan F (2010) Application of SYBR green PCR and 2DGE methods to authenticate edible bird’s nest food. Food Res Int 43:2020-026 CrossRef
  2. Goh DLM, Chua KY, Chew FT, Seow TK, Ou KL, Yi FC, Lee BW (2001) Immunochemical characterization of edible bird’s nest allergens. J Allergy Clin Immun 107:1082-088 CrossRef
  3. Lin JR, Zhou H, Lai XP, Hou Y, Xian XM, Chen JN, Wang PX, Zhou L, Dong Y (2009) Genetic identification of edible bird’s nest based on mitochondrial DNA sequences. Food Res Int 42:1053-061 CrossRef
  4. Matsukawa N, Matsumoto M, Bukawa W, Chiji H, Nakayama K, Hara H, Tsukahara T (2011) Improvement of bone strength and dermal thickness due to dietary edible bird’s nest extract in ovariectomized rats. Biosci Biotech Bioch 75:590-92 CrossRef
  5. Guo CT, Takahashi T, Bukawa W, Takahashi N, Yagi H, Kato K, Hidari KL, Miyamoto D, Suzuki T, Suzuki Y (2006) Edible bird’s nest extract inhibits influenza virus infection. Antivir Res 70:140-46 CrossRef
  6. Schauer R, Shula AK, Schr?der C, Müller E (1984) The anti-recognition function of sialic acids: studied with erythrocytes and macrophages. Pure Appl Chem 56:907-21 CrossRef
  7. Houdret N, Lhermitte M, Degand P, Roussel P (1975) Purification and chemical study of a Collocalia glycoprotein. Biochimie 57:603-08 CrossRef
  8. Martin JE, Tanenbaum SW, Flashner M (1977) A facile procedure for the isolation of / N-acetylneuramic acid from edible bird’s nest. Carbohydr Res 56:423-25 CrossRef
  9. Yagi H, Yasukawa N, Yu SY, Guo CT, Takahashi N, Takahashi T, Bukawa W, Suzuki T, Khoo KH, Suzuki Y, Kato K (2008) The expression of sialylated high-antennary / N-glycans in edible bird’s nest. Carbohydr Res 343:1373-377 CrossRef
  10. Zhang SW, Lai XT, Liu XQ, Li Y, Li BF, Huang XL, Zhang QL, Chen W, Lin L, Yang GW (2012) Competitive enzyme-linked immunoassay for sialoglycoprotein of edible bird’s nest in food and cosmetics. J Agric Food Chem 60:3580-585 CrossRef
  11. Ma FC, Liu DC (2012) Sketch of the edible bird’s nest and its important bioactivities. Food Res Int 48:559-67 CrossRef
  12. Oda M (1983) Saccharides and amino acids of the mucoid in an edible bird’s nest. Nippon Shokuhin Kogyo Gakkaishi 30:221-27 CrossRef
  13. Tong TJ, Liu DY (1985) Isolation and identification of glycopeptide in edible bird’s nest. Chin Sci Bull 12:949-52
  14. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265-75
  15. Masuko T, Minami A, Iwasaki N, Majima T, Nishimura SI, Lee YC (2005) Carbohydrate analysis by a phenol-sulfuric acid method in microplate format. Anal Biochem 339:69-2 CrossRef
  16. Geisler C, Jarvis DJ (2011) Effective glycoanalysis with / Maackia amurensis lectins requires a clear understanding of their binding specificities. Glycobiology 21:988-93 CrossRef
  17. Wang WC, Cummings RD (1988) The immobilized leukoagglutinin from the seeds of / Maackia amurensis binds with high affinity to complex-type Asn-linked oligosaccharides containing terminal sialic acid-linked / α-2, 3 to penultimate galactose residues. J Biol Chem 263:4576-585
  18. Bai XM, Brown JR, Varki A, Esko JD (2001) Enhanced 3- / O-sulfa
• 作者单位：Yanyan You (1)
  Yan Cao (1)
  Shuang Guo (1)
  Jie Xu (1)
  Zhaojie Li (1)
  Jingfeng Wang (1)
  Changhu Xue (1)
  
  1. College of Food Science and Engineering, Ocean University of China, No. 5, Yu Shan Road, Qingdao, 266003, Shandong Province, People’s Republic of China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Food Science
  Analytical Chemistry
  Biotechnology
  Agriculture
  Forestry
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1438-2385

文摘

The distribution of α 2- and α 2- linked sialoglycoproteins in edible bird’s nest (EBN) was analyzed by Maackia amurensis agglutinin (MAA) and Sambucus nigra agglutinin (SNA) lectin blotting. Q Sepharose Fast Flow, Superdex 75 and Sephadex G-25 columns were combined to enrich sialoglycoproteins from EBN extraction. For purification and identification of α 2- linked sialoglycoprotein and α 2- linked sialoglycoprotein, MAA-Sepharose-4B, SNA-Sepharose-4B lectin affinity chromatography and matrix-assisted laser desorption–ionization time-of-flight/time-of-flight (MALDI-TOF/TOF) tandem mass spectrometry (MS) were employed successively. The contents of protein, carbohydrate and sialic acid of EBN extraction were 65.43, 20.97 and 10.47?%, respectively; EBN extraction contained high-abundance glycoproteins with the molecular weights of 128?kDa (19.1?%), 106?kDa (18.5?%) and 43?kDa (29.4?%); the 43?kDa glycoproteins contained terminal α 2- and α 2- sialic acid linkage were successively enriched by three chromatography columns; α 2- linked sialoglycoprotein and α 2- linked sialoglycoprotein were further purified by affinity chromatography eluted with 0.3 and 0.5?M lactose, respectively. In addition, MALDI-TOF/TOF MS analysis showed that α 2- linked sialoglycoprotein was an acidic mammalian chitinase-like protein, and α 2- linked sialoglycoprotein was an acidic mammalian chitinase. This method is proved to be a simple and effective approach to purify sialoglycoproteins from high-abundance glycoproteins in EBN.