Isolation and Immobilization of Influenza Virus-Specific N-SA-α-2,3-Gal Receptors Using Magnetic Nanoparticles Coated with Chitosan and Maackia amurensis Lectin

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• 作者：Susana Araceli Carrizales álvarez (1)
  Anna Ilyina (1)
  Karla Monzerratt Gregorio Jáuregui (2)
  José Luis Martínez Hernández (1)
  Brenda Berenice Vazquez Gutiérrez (1)
  Elda Patricia Segura Ceniceros (1)
  Alejandro Zugasti Cruz (1)
  Hened Saade Caballero (3)
  Raúl Guillermo López Campos (3)
  
• 关键词：Maackia amurensis lectin ; N ; SA ; α ; 2 ; 3 ; Gal ; linked receptors ; Chitosan ; coated magnetic nanoparticles
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2014
• 出版时间：November 2014
• 年：2014
• 卷：174
• 期：5
• 页码：1945-1958
• 全文大小：706 KB
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• 作者单位：Susana Araceli Carrizales álvarez (1)
  Anna Ilyina (1)
  Karla Monzerratt Gregorio Jáuregui (2)
  José Luis Martínez Hernández (1)
  Brenda Berenice Vazquez Gutiérrez (1)
  Elda Patricia Segura Ceniceros (1)
  Alejandro Zugasti Cruz (1)
  Hened Saade Caballero (3)
  Raúl Guillermo López Campos (3)
  
  1. Facultad de Ciencias Químicas, Grupo de Nanobiociencia de la Facultad de Ciencias Químicas de la Universidad Autónoma de Coahuila, Blvd. V. Carranza e Ing. José Cárdenas V. Col. República Oriente, C.P. 25280, Saltillo, Coahuila, Mexico
  2. Escuela Superior de Ingeniería Química e Industrias Extractivas, UPALM, Instituto Politécnico Nacional, Col. San Pedro Zacatenco, C.P. 07738, México, D.F., Mexico
  3. Departamento de Procesos de Polimerización, Centro de Investigación en Química Aplicada, Coahuila, Blvd. Enrique Reyna Hermosillo 140, C.P. 25290, Saltillo, Coahuila, Mexico
  
• ISSN：1559-0291

文摘

Avian influenza viruses preferentially bind to sialic acid alpha-2,3-galactose (N-SA-α-2,3-Gal) receptors on epithelial cells. Herein, we describe a procedure we have developed for isolation of N-SA-α-2,3-Gal receptors from porcine trachea using magnetic nanoparticles (NPs) coated with chitosan (NP-Ch) and functionalized with Maackia amurensis lectin (NP-lectin). Magnetic nanoparticles were coated with chitosan in a one-step co-precipitation, and then M. amurensis lectin was immobilized covalently using glutaraldehyde. Lectin?coated nanoparticles were incubated with sialic acid?enriched fraction of tracheal homogenate, and N-SA-α-2,3-Gal receptor was extracted under magnetic field in two cycles. The presence of 66.4?kDa protein was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The interaction of immobilized receptor (NP-Ch-R) with M. amurensis lectin (NP-Ch-R-L) was demonstrated by Fourier transform infrared spectrometry (FTIR) and thermogravimetric analysis (TGA).