Effects of cell surface α2-3 sialic acid on osteogenesis

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• 作者：Lan Xu ; Wei Xu ; Guiying Xu ; Zhi Jiang ; Lei Zheng ; Yinghui Zhou…
• 关键词：MC3T3 ; E1 subclone 14 ; Osteoblasts ; α2 ; 3 sialic acid ; α2 ; 3 ; neuraminidase
• 刊名：Glycoconjugate Journal
• 出版年：2013
• 出版时间：October 2013
• 年：2013
• 卷：30
• 期：7
• 页码：677-685
• 全文大小：899KB
• 参考文献：1. Varki, N.M., Varki, A.: Diversity in cell surface sialic acid presentations: implications for biology and disease. Lab. Investig. 87, 851-57 (2007) CrossRef
  2. Meesmann, H.M., Fehr, E.M., Kierschke, S., Herrm, M., Bilyy, R., Heyde, P., Blan, N., Krienke, S., Loren, H.M., Schiller, M.: Decrease of sialic acid residues as an eat-me signal on the surface of apoptotic lymphocytes. J. Cell. Sci. 123, 3347-356 (2010) CrossRef
  3. Stanley, P., Cummings, R.S.: Structures common to different glycans. In: Varki, A., Cummings, R.D., Esko, J.D., Freeze, H.H., Stanley, P., Bertozzi, C.R., Hart, G.W., Etzler, M.E. (eds.) Essentials of Glycobiology. Cold Spring Harbor Laboratory Press, New York (2009)
  4. Mathieu, S., Prorok, M., Benoliel, A.M., Uch, R., Langlet, C., Bongrand, P., Gerolami, R., El-Battari, A.: Transgene expression of alpha(1,2)-fucosyltransferase-I (FUT1) in tumor cells selectively inhibits sialyl-Lewis x expression and binding to E-selectin without affecting synthesis of sialyl-Lewis a or binding to P-selectin. Am. J. Pathol. 164, 371-83 (2004) CrossRef
  5. Kannagi, R.: Molecular mechanism for cancer-associated induction of sialyl Lewis X and sialyl Lewis A expression—the Warburg effect revisited. Glycoconj. J. 20, 353-64 (2004) CrossRef
  6. Li, W., Ding, Q.W., Jiang, Z., Xu, L., Wu, S.L.: A preliminary study on the effects of membrane α2,3 polysialic acid residues on human gastric cancer cell AGS’s growth and migration and several related genes-expression. Chin. J. Hemorheol. 3, 353-55 (2010)
  7. Taipaleenm?ki, H., Bjerre Hokland, L., Chen, L., Kauppinen, S., Kassem, M.: Mechanisms in endocrinology: micro-RNAs: targets for enhancing osteoblast differentiation and bone formation. Eur. J. Endocrinol. 166, 359-71 (2012) CrossRef
  8. Wang, J., Glimcher, M.J., Mah, J., Zhou, H.Y., Salih, E.: Expression of bone microsomal casin Kinas II, bone sialoprotein and osteopontin during the repair of calvarial defects. Bone 22, 621-28 (1998) CrossRef
  9. Bancroft, J.D., Alan, S.: A Theory and Practice of Histological Techniques, 4th edn, pp. 309-39. Churchill Livingstones, New York (1996)
  10. Manolagas, S.C., Jilka, R.L.: Bone marrow, cytokines and bone remodeling. Emerging insights into the pathophysiology of osteoporosis. N. Engl. J. Med. 332, 305-11 (1995) CrossRef
  11. Zheng, M.H., Wood, D.J., Papadimitrion, J.M.: What’s new in the role of cytokines on osteoblast proliferation and differentiation. Pathol. Res. Pract. 188, 1104-121 (1992) CrossRef
  12. Wang, D., Christensen, K., Chawla, K., Xiao, G., Krebsbach, P.H., Franceschi, R.T.: Isolation and characterization of MC3T3-E1 preosteoblast subclones with distinct / in vitro and / in vivo differentiation/mineralization potential. J. Bone Miner. Res. 14, 893-03 (1999) CrossRef
  13. Chenc, S.L., Zhang, S.F., Mohan, S., Lecanda, F., Fausto, A., Hunt, A.H., Canalis, E., Avioli, L.V.: Regulation of insulin-like growth factors Iand IIand their binding proteins in human bone marrow stromal cells by dexamethasone. J. Cell. Biochem. 71, 449-58 (1998) CrossRef
  14. Maniatopoulos, C., Sodek, J., Melcher, A.H.: Bone formation / in vitro by stromal cell obtained from bone marrow of young adult rat. Cell Tissue Res. 254, 317-30 (1998)
  15. Peter, H.S.: Exploring life’s sweet spot. Nature 437, 1239 (2005) CrossRef
  16. Lloyd, R.C., Davis, B.G., Jones, J.B.: Site-selective glycosylation of subtilisin bacillus lentus cause dramatic increases in esterase activity. Bioorg. Med. Chem. 8, 1537-544 (2000) CrossRef
  17. Wuttke, M., Muller, S., Nitsche, D.P., Paulsson, M., Hanisch, F.G., Maurer, P.: Structural characterization of human recombinant and bone-derived bone sialoprotein. Functional implications for cell attachment and hydroxyapatite binding. J. Biol. Chem. 276, 36839-6848 (2001) CrossRef
  18. Zhang, W.G., Wang, L.Z., Liu, Z.: The effect of fluoride on bone extracellular matrix proteins expression in rat calvarial osteoblast in culture. Shanghai J. Stomatol. 7(103), 94-8 (1998)
  19. Lian, J., Stewart, C., Puchacz, E., Mackowiak, S., Shalhoub, V., Collart, D., Zambetti, G., Stein, G.: Structure of the rat osteocalcin gene and regulation of vitamin D-dependent expression. Proc. Natl. Acad. Sci. U. S. A. 86, 1143-147 (1989) CrossRef
  20. Lucotte, G., Mercier, G., Birckel, A.: The vitamin D receptor FokI start coden polymorphism and bone mineral density in osteoporotic postmenopausal French women. Clin. Genet. 56(3), 221-24 (1999) CrossRef
  21. Nguyen, T.V., Kelly, P.J., Morrison, N.A.: Vitamin D receptor genotypesin osteoporosis. Lancet 344(8936), 1580-581 (1994)
  
• 作者单位：Lan Xu (1)
  Wei Xu (2)
  Guiying Xu (1)
  Zhi Jiang (1)
  Lei Zheng (1)
  Yinghui Zhou (1)
  Wenxiang Wei (3) (4)
  Shiliang Wu (1)
  
  1. Department of Biochemistry and Molecular Biology, School of Medicine, Soochow University, Suzhou, 215007, China
  2. Division of Orthopaedic Surgery, The Second Affiliated Hospital, School of Medicine, Soochow University, Suzhou, 215007, China
  3. Department of Cell Biology, School of Medicine, Soochow University, Suzhou, 215007, China
  4. Department of Cell Biology, School of Medicine, Soochow University, Suzhou, 215123, China
  
• ISSN：1573-4986

文摘

A cell culture model of osteoblast differentiation was applied in our study of the effect of sialic acid on the osteogenesis by using the pre-osteoblast of MC3T3-E1 subclone 14 cells. Following the treatment of different concentrations of α2,3-neuraminidase, which specifically removed the α2-3 sialic acid from cell surface, a significant decrease of α2-3 sialic acid was detected with fluorescein isothiocyanate (FITC)-labeled Maackia amurensis lectin (MAL-II) by flow cytometry analysis. von Kossa staining showed that the bone mineralization decreased in MC3T3-E1 subclone 14 cells after the treatment of α2,3-neuraminidase for 2?weeks. However α2,3-neuraminidase did not affect the formation of osteoblasts in MC3T3-E1 subclone 14 cells, which was demonstrated by positive alkaline phosphatase (ALP)-staining. Characteristic biological markers and osteoblast-like cell-related factors of osteoblastic cells were also examined. Both RT-PCR and Western blot analysis demonstrated that the expression of bone sialoprotein (BSP), osteoprotegerin (OPG), and vitamin D receptor (VDR) were significantly decreased when α2-3 sialic acid expression decreased on the cell surface, while the expression of osteocalcin (OC) and osteopontin (OPN) remained unchanged. We propose a hypothesis that α2-3 sialic acid affects bone mineralization but not osteogenic differentiation.