Antimicrobial activity of Bacillus amyloliquefaciens EMD17 isolated from Cheonggukjang and potential use as a starter for fermented soy foods

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• 作者：Jae Yong Lee ; Jae Min Shim ; Zhuang Yao ; Xiaoming Liu…
• 关键词：Bacillus amyloliquefaciens ; antimicrobial activity ; lipopeptide ; surfactin ; starter
• 刊名：Food Science and Biotechnology
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：25
• 期：2
• 页码：525-532
• 全文大小：440 KB
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• 作者单位：Jae Yong Lee (1)
  Jae Min Shim (1)
  Zhuang Yao (1)
  Xiaoming Liu (1)
  Kang Wook Lee (1)
  Hyun-Jin Kim (1) (3)
  Kyung-Sik Ham (2)
  Jeong Hwan Kim (1) (3)
  
  1. Division of Applied Life Science (BK21 plus), Graduate School, Gyeongsang National University, Jinju, Gyeongnam, 52828, Korea
  3. Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, Gyeongnam, 52828, Korea
  2. Department of Food Engineering and Solar Salt Research Center, Mokpo National University, Muan, Jeonnam, 58554, Korea
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Nutrition
  Food Science
  
• 出版者：The Korean Society of Food Science and Technology in co-publication with Springer
• ISSN：2092-6456

文摘

A Bacillus strain with antimicrobial activity was isolated from Cheonggukjang and identified as Bacillus amyloliquefaciens. Filtered culture supernatants of B. amyloliquefaciens EMD17 strongly inhibited growth of Bacillus cereus ATCC14579, Listeria monocytogenes ATCC19111, and an ochratoxin (OTA) producing Penicillium spp. The antimicrobial activity was not decreased by heat, pH, and proteases treatment, indicating a non-proteinous nature of the antimicrobial substance. A B. amyloliquefaciens EMD17 culture added to a B. cereus ATCC14579 culture killed B. cereus cells completely in 6 h, showing the bacteriocidal effect. The srfAA gene encoding surfactin synthetase A was detected using PCR, indicating that surfactin might be the responsible agent. Genes encoding fengycin and iturin were also detected. MS indicated production of lipopeptides, including surfactin. Cheonggukjang and Doenjang were prepared using B. amyloliquefaciens EMD17 as a starter and growth of spiked B. cereus cells was completely inhibited.