Molecular characterization and expression analysis of osteopontin cDNA from lactating mammary gland in yak (Bos grunniens)

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• 作者：W. L. Bai (1)
  R. J. Yang (5)
  R. H. Yin (1) yinronghuan@163.com
  W. Q. Jiang (1)
  G. B. Luo (1)
  R. L. Yin (2)
  S. J. Zhao (3)
  C. Li (4)
  Z. H. Zhao (5) zh65zh@yahoo.cn
• 关键词：Yak &#8211 ; Osteopontin cDNA &#8211 ; Phylogenetic analysis &#8211 ; Expression analysis
• 刊名：Molecular Biology Reports
• 出版年：2012
• 出版时间：April 2012
• 年：2012
• 卷：39
• 期：4
• 页码：3627-3635
• 全文大小：433.2 KB
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• 作者单位：1. College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866 China2. Research Academy of Animal Husbandry and Veterinary Medicine Science of Jilin Province, Changchun, 130062 China3. Institute of Biotechnology, Animal Science Research Academy of Sichuan Province, Chengdu, 610066 China4. Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, 130062 China5. College of Animal Science and Veterinary Medicine, Jilin University, Changchun, 130062 China
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Animal Anatomy, Morphology and Histology
  Animal Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-4978

文摘

Osteopontin (OPN) is a secreted phosphorylated glycoprotein. It has an important role in mammary gland development and lactation, as well as, is thought to be a potential candidate gene for lactation traits. In the present work, we isolated and characterized a full-length open reading frame (ORF) of yak OPN cDNA from lactating mammary tissue, and examined its expression pattern in mammary gland during different stages of lactation, as well as, the recombinant OPN protein of yak was expressed successfully in E. coli. The sequencing results indicated that the isolated cDNA was 1132-bp in length containing a complete ORF of 837-bp. It encoded a precursor protein of yak OPN consisting of 278 amino acid with a signal peptide of 16 amino acids. Yak OPN has a predicted molecular mass of 29285.975 Da and an isoelectric point of 4.245. It had an identity of 65.50–99.16% in cDNA, identity of 52.06–98.56% and similarity of 65.40–98.56% in deduced amino acids with the corresponding sequences of cattle, buffalo, sheep, goat, pig, human, and rabbit. The phylogenetic analysis indicated that yak OPN had the closest evolutionary relationship with that of cattle, and next buffalo. In mammary gland, yak OPN was generally transcribed in a declining pattern from colostrum period to dry period with an apparent increase of OPN expression being present in the late period of lactation compared with peak period of lactation. Western blot analysis indicated that His-tagged yak OPN protein expressed in E. coli could be recognized not only by an anti-His-tag antibody but also by an anti-human OPN antibody. These results from the present work provided a foundation for further insight into the role of OPN gene in yak lactation.