Fatty acid content in epididymal fluid and spermatozoa during sperm maturation in dogs
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- 作者：Daniel S. Ramos Angrimani ; Marcilio Nichi…
- 关键词：Dogs ; Epididymis ; Fatty acids ; Sperm maturation
- 刊名：Journal of Animal Science and Biotechnology
- 出版年：2017
- 出版时间：December 2017
- 年：2017
- 卷：8
- 期：1
- 全文大小：515KB
- 刊物主题：Agriculture; Biotechnology; Food Science; Animal Genetics and Genomics; Animal Physiology;
- 出版者：BioMed Central
- ISSN：2049-1891
- 卷排序：8
文摘
BackgroundDuring sperm maturation, there is a reorganization of fatty acids from plasmatic membrane of the spermatozoa, which allows higher membrane integrity and acquisition of sperm motility. However, the fatty acid profile during sperm maturation remains unclear in dogs. Thus, the aim of this study was to identify the fatty acids from the epididymal spermatozoa and plasma during the sperm maturation, and observed changes in the motility and plasmatic membrane parameters. Twenty one adult dogs were used, subsequently to bilateral orchiectomy and epididymal storage, sperm samples were collected from the different segments of the epididymis. Samples were evaluated for conventional microscopy, computer-assisted motility analysis, sperm plasma membrane permeability and the fatty acid analysis (lipids were extracted, transmethylated and analyzed by chromatography).