Screening of liquid media and fermentation of an endophytic Beauveria bassiana strain in a bioreactor

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• 作者：Rieke Lohse ; Desiree Jakobs-Sch?nwandt ; Anant V Patel
• 关键词：Submerged culture ; Fermentation ; Beauveria bassiana ; Endophyte ; Blastospores ; Submerged conidiospores ; Biological control
• 刊名：AMB Express
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：4
• 期：1
• 全文大小：973 KB
• 参考文献：1. Akello, J, Dubois, T, Coyne, D, Kyamanywa, S (2008) Endophytic Beauveria bassiana in banana (Musa spp.) reduces banana weevil (Cosmopolites sordidus) fitness and damage. Crop Prot 27: pp. 1437-1441 blank" title="It opens in new window">CrossRef <br> 2. Bidochka, MJ, Khachatourians, GG (1993) Oxalic acid hyperproduction in Beauveria bassiana mutants is related to a utilizable carbon source but not to virulence. J Invertebr Pathol 62: pp. 53-57 blank" title="It opens in new window">CrossRef <br> 3. Bidochka, MJ, Pfeifer, TA, Khachatourians, GG (1987) Development of the entomopathogenic fungus Beauveria bassiana in liquid cultures. Mycopathologia 99: pp. 77-83 blank" title="It opens in new window">CrossRef <br> 4. Bing, LA, Lewis, LC (1991) Suppression of Ostrinia nubilalis (Hubner)(Lepidoptera: pyralidae) by endophytic Beauveria bassiana (Balsamo) Vuillemin. Environ Entomol 20: pp. 1207-1211 <br> 5. Bing, LA, Lewis, LC (1992) Endophytic Beauveria bassiana (Balsamo) Vuillemin in corn: the influence of the plant growth stage and Ostrinia nubilalis (Hübner). Biocontrol Sci Techn 1: pp. 29-47 <br> 6. Burges, HD (1998) Formulation of microbial pesticides. Kluwer Academic Publishers, Dordrecht, The Netherlands blank" title="It opens in new window">CrossRef <br> 7. Chase, AR, Osborne, LS, Ferguson, VM (1986) Selective isolation of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae from an artificial potting medium. Fla Entomol 69: pp. 285-292 blank" title="It opens in new window">CrossRef <br> 8. Chong-Rodríguez, MJ, Maldonado-Blanco, MG, Hernández-Escare?o, JJ, Galán-Wong, LJ, Sandoval-Coronado, CF (2011) Study of Beauveria bassiana growth, blastospore yield, desiccation-tolerance, viability and toxic activity using different liquid media. Afr J Biotechnol 10: pp. 5736-5742 <br> 9. Fargues, J, Reisinger, O, Robert, PH, Aubart, C (1983) Biodegradation of entomopathogenic hyphomycetes: influence of clay coating on Beauveria bassiana blastospore survival in soil. J Invertebr Pathol 41: pp. 131-142 blank" title="It opens in new window">CrossRef <br> 10. Feng, MG, Poprawski, TJ, Khachatourians, GG (1994) Production, formulation and application of the entomopathogenic fungus Beauveria bassiana for insect control: current status. Biocontrol Sci Techn 4: pp. 3-34 blank" title="It opens in new window">CrossRef <br> 11. Glare, T, Caradus, J, Gelernter, W, Jackson, T, Keyhani, N, K?hl, J, Marrone, P, Morin, L, Stewart, A (2012) Have biopesticides come of age?. Trends Biotechnol 30: pp. 250-258 btech.2012.01.003" target="_blank" title="It opens in new window">CrossRef <br> 12. Godonou, I, James, B, Atcha-Ahowé, C, Vodouhè, S, Kooyman, C, Ahanchédé, A, Korie, S (2009) Potential of Beauveria bassiana and Metarhizium anisopliae isolates from Benin to control Plutella xylostella L. (Lepidoptera: plutellidae). Crop Prot 28: pp. 220-224 blank" title="It opens in new window">CrossRef <br> 13. Gurulingappa, P, Sword, GA, Murdoch, G, McGee, PA (2010) Colonization of crop plants by fungal entomopathogens and their effects on two insect pests when in planta. Biol Control 55: pp. 34-41 biocontrol.2010.06.011" target="_blank" title="It opens in new window">CrossRef <br> 14. Hall, RA, Papierok, B (1982) Fungi as biological control agents of arthropods of agricultural and medical importance. Parasitology 84: pp. 205-240 blank" title="It opens in new window">CrossRef <br> 15. Hardoim, PR, Overbeek, LS, Elsas, JD (2008) Properties of bacterial endophytes and their proposed role in plant growth. Trends Microbiol 16: pp. 463-471 blank" title="It opens in new window">CrossRef <br> 16. Hegedus, DD, Bidochka, MJ, Khachatourians, GG (1990) Beauveria bassiana submerged conidia production in a defined medium containing chitin, two hexosamines or glucose. Appl Microbiol Biotechnol 33: pp. 641-647 blank" title="It opens in new window">CrossRef <br> 17. Hegedus, DD, Bidochka, MJ, Miranpuri, GS, Khachatourians, GG (1992) A com
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry<br>Biotechnology<br>Microbiology<br>Microbial Genetics and Genomics<br>
• 出版者：Springer Berlin / Heidelberg
• ISSN：2191-0855

文摘

A novel approach for biological control of insect pests could be the use of the endophytic entomopathogenic Beauveria bassiana isolate ATP-02. For the utilization of the endophyte as a commercial biocontrol agent, the fungus has to be mass-produced. B. bassiana was raised in shake flask cultures to produce high concentrations of total spores (TS), which include blastospores (BS) and submerged conidiospores (SCS). The highest concentration of 1.33×109 TS/mL and the highest yield of 5.32×1010 TS/g sucrose was obtained in the TKI broth with 5% sugar beet molasses which consists of 50% sucrose as a carbon source. In spite of the lower sugar concentration (2.5%) the amount of TS could be increased up to 11-times in contrast to the cultivation with 5% sucrose. The scale-up to a 2?L stirred tank reactor was carried out at 25°C, 200-00?rpm and 1 vvm at pH?5.5. A TS yield of 5.2×1010 TS/g sucrose corresponding to a SCS yield of 0.2×1010 SCS/g sucrose was obtained after 216?h. With regards to the culture medium the cost of 1012 TS amounts to 0.24 ? Plutella xylostella larvae, which were fed with oilseed rape leaves treated with spores from fermentation resulted in 77?±-% mortality. Moreover, spores from submerged cultivation were able to colonize oilseed rape leaves via leaf application. This is the first report of fermentation of an endophytic B. bassiana strain in a low-cost culture medium to very high yields of TS.