Ghrelin and NUCB2/nesfatin-1 are expressed in the same gastric cell and differentially correlated with body mass index in obese subjects

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• 作者：Andreas Stengel (1)
  Tobias Hofmann (1)
  Miriam Goebel-Stengel (2)
  Vanessa Lembke (1)
  Anne Ahnis (1)
  Ulf Elbelt (3)
  Nils W. G. Lambrecht (4)
  Jürgen Ordemann (5)
  Burghard F. Klapp (1)
  Peter Kobelt (1)
  
• 关键词：Bariatric surgery ; GOAT ; Hormone ; Nesfatin ; 1 ; Stomach ; X/A ; like cell
• 刊名：Histochemistry and Cell Biology
• 出版年：2013
• 出版时间：June 2013
• 年：2013
• 卷：139
• 期：6
• 页码：909-918
• 全文大小：843KB
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• 作者单位：Andreas Stengel (1)
  Tobias Hofmann (1)
  Miriam Goebel-Stengel (2)
  Vanessa Lembke (1)
  Anne Ahnis (1)
  Ulf Elbelt (3)
  Nils W. G. Lambrecht (4)
  Jürgen Ordemann (5)
  Burghard F. Klapp (1)
  Peter Kobelt (1)
  
  1. Charité Center for Internal Medicine and Dermatology, Division Psychosomatic Medicine, Obesity Center Berlin, Charité-Universit?tsmedizin Berlin, Campus Mitte, Charitéplatz 1, 10117, Berlin, Germany
  2. Department of Internal Medicine, Institute of Neurogastroenterology and Motility, Martin-Luther Hospital, Academic Teaching Institution of Charité-Universit?tsmedizin, Berlin, Germany
  3. Division Endocrinology, Department of Medicine, Diabetes and Nutrition, Obesity Center Berlin, Charité-Universit?tsmedizin Berlin, Campus Mitte, Berlin, Germany
  4. Gastrointestinal Endocrinology, Veterans Affairs Long Beach Healthcare System, Long Beach, CA, USA
  5. Center for Obesity and Metabolic Surgery, Charité-Universit?tsmedizin Berlin, Campus Mitte, Berlin, Germany
  
• ISSN：1432-119X

文摘

The orexigenic peptide ghrelin and the anorexigenic peptide nesfatin-1 are expressed by the same endocrine cell of the rat stomach, the X/A-like cell. However, data in humans are lacking, especially under conditions of obesity. We collected gastric tissue of obese patients undergoing sleeve gastrectomy and investigated the expression of nesfatin-1 and ghrelin in the gastric oxyntic mucosa by immunofluorescence. Nesfatin-1 immunoreactivity was detected in the human oxyntic mucosa in cells with an endocrine phenotype. A major portion of nesfatin-1 immunoreactive cells (78?%) co-localized with ghrelin indicating the occurrence in human X/A-like cells. In patients with very high body mass index (BMI 55-5?kg/m2), the number of nesfatin-1 immunoreactive cells/low-power field was significantly higher than in obese patients with lower BMI (40-0?kg/m2, 118?±?10 vs. 82?±?11, p?<?0.05). On the other hand, the number of ghrelin immunoreactive cells was significantly reduced in obese patients with higher compared to lower BMI (96?±?12 vs. 204?±?21, p?<?0.01). Also the ghrelin-acylating enzyme ghrelin-O-acyltransferase decreased with increasing BMI. In conclusion, nesfatin-1 immunoreactivity is also co-localized with ghrelin in human gastric X/A-like cells giving rise to a dual role of this cell type with differential effects on stimulation and inhibition of appetite dependent on the peptide released. The expression of these two peptides is differentially regulated under obese conditions with an increase of nesfatin-1 and a decrease of ghrelin immunoreactivity with rising BMI pointing towards an adaptive change of expression that may counteract further body weight increase.