An R2R3 MYB transcription factor determines red petal colour in an Actinidia (kiwifruit) hybrid population
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- 作者：Lena G Fraser (1)
Alan G Seal (2)
Mirco Montefiori (1)
Tony K McGhie (3)
Gianna K Tsang (1)
Paul M Datson (1)
Elena Hilario (1)
Hinga E Marsh (2)
Juanita K Dunn (2)
Roger P Hellens (1)
Kevin M Davies (3)
Mark A McNeilage (1)
H Nihal De Silva (1)
Andrew C Allan (1) - 关键词：Actinidia ; Kiwifruit ; Anthocyanin ; MYB ; Transcription factor ; Colour
- 刊名：BMC Genomics
- 出版年：2013
- 出版时间：December 2013
- 年：2013
- 卷：14
- 期：1
- 全文大小：2304KB
- 参考文献：1. Gould K, Lister C:
2. Grotewold E:
3. de Pascual-Teresa S, Sanchez-Ballesta MT:
4. Kay CD:
5. Stevenson DE, Hurst RD:
6. Zafra-Stone S, Yasmin T, Bagchi M, Chatterjee A, Vinson JA, Bagchi D:
7. Baudry A, Heim MA, Dubreucq B, Caboche M, Weisshaar B, Lepiniec L:
8. Koes R, Verweij W, Quattrocchio F:
9. Lepiniec L, Debeaujon I, Routaboul J-M, Baudry A, Pourcel L, Nesi N, Caboche M:
10. Aharoni A, De Vos CHR, Wein M, Sun ZK, Greco R, Kroon A, Mol JNM, O'Connell AP:
11. Albert NW, Lewis DH, Zhang H, Schwinn KE, Jameson PE, Davies KM:
12. Dubos C, Le Gourrierec J, Baudry A, Huep G, Lanet E, Debeaujon I, Routaboul J-M, Alboresi A, Weisshaar B, Lepiniec L:
13. Lin-Wang K, Micheletti D, Palmer J, Volz R, Lozano L, Espley R, Hellens RP, Chagne D, Rowan DD, Troggio M:
14. Matsui K, Umemura Y, Ohme-Takagi M:
15. Allan AC, Hellens RP, Laing WA:
16. Schwinn K, Venail J, Shang YJ, Mackay S, Alm V, Butelli E, Oyama R, Bailey P, Davies K, Martin C:
17. Feller A, Machemer K, Braun EL, Grotewold E:
18. Gonzalez A, Zhao M, Leavitt JM, Lloyd AM:
19. Martin C, Ellis N, Rook F:
20. Quattrocchio F, Wing J, van der Woude K, Souer E, de Vetten N, Mol J, Koes R:
21. Cui Z, Huang H, Xiao X: / Actinidia in China. Beijing: China Agricultural Science and Technology Press; 2002.
22. Wang SM, Huang HW, Jiang ZW, Zhang ZH, Zhang SR, Huang HQ:
23. Xiong ZT, Wang SM, Huang RH:
24. Zhang L, Li Z, Wang Y, Jiang Z, Wang S, Huang H:
25. Fan P, An H, Cai D, Mu X, Fan PG, An HX, Cai DR, Mu XJ:
26. Crowhurst RN, Gleave AP, MacRae EA, Ampomah-Dwamena C, Atkinson RG, Beuning LL, Bulley SM, Chagne D, Marsh KB, Matich AJ:
27. Lin-Wang K, Bolitho K, Grafton K, Kortstee A, Karunairetnam S, McGhie TK, Espley RV, Hellens RP, Allan AC:
28. Zimmermann IM, Heim MA, Weisshaar B, Uhrig JF:
29. Grotewold E, Sainz MB, Tagliani L, Hernandez JM, Bowen B, Chandler VL:
30. Fraser LG, Tsang GK, Datson PM, De Silva HN, Harvey CF, Gill GP, Crowhurst RN, McNeilage MA:
31. Montefiori M, Espley RV, Stevenson D, Cooney J, Datson PM, Saiz A, Atkinson RG, Hellens RP, Allan AC:
32. Espley RV, Hellens RP, Putterill J, Stevenson DE, Kutty-Amma S, Allan AC:
33. Comeskey DJ, Montefiori M, Edwards PJB, McGhie TK:
34. Montefiori M, Comeskey DJ, Wohlers M, McGhie TK:
35. Castaneda-Ovando A, de Lourdes P-HM, Elena Paez-Hernandez M, Rodriguez JA, Andres Galan-Vidal C:
36. Davies AJ, Mazza G:
37. Johnson ET, Yi HK, Shin BC, Oh BJ, Cheong HS, Choi G:
38. Kumar S, Tamura K, Nei M:
39. Hellens RP, Allan AC, Friel EN, Bolitho K, Grafton K, Templeton MD, Karunairetnam S, Gleave AP, Laing WA:
40. Gplots: / Various R programming tools for plotting data. R package version 2.10.0. g/package=gplots" class="a-plus-plus">http://CRAN.Rproject.org/package=gplots - 作者单位：Lena G Fraser (1)
Alan G Seal (2)
Mirco Montefiori (1)
Tony K McGhie (3)
Gianna K Tsang (1)
Paul M Datson (1)
Elena Hilario (1)
Hinga E Marsh (2)
Juanita K Dunn (2)
Roger P Hellens (1)
Kevin M Davies (3)
Mark A McNeilage (1)
H Nihal De Silva (1)
Andrew C Allan (1)
1. The New Zealand Institute for Plant & Food Research Limited, 120 Mt. Albert Road, Auckland, 1142, New Zealand
2. The New Zealand Institute for Plant & Food Research Limited, 412 No. 1 Road, RD 2, Te Puke, 3182, New Zealand
3. The New Zealand Institute for Plant & Food Research Limited, Fitzherbert Science Centre, Batchelar Road, Palmerston North, 4474, New Zealand
文摘
Background Red colour in kiwifruit results from the presence of anthocyanin pigments. Their expression, however, is complex, and varies among genotypes, species, tissues and environments. An understanding of the biosynthesis, physiology and genetics of the anthocyanins involved, and the control of their expression in different tissues, is required. A complex, the MBW complex, consisting of R2R3-MYB and bHLH transcription factors together with a WD-repeat protein, activates anthocyanin 3-O-galactosyltransferase (F3GT1) to produce anthocyanins. We examined the expression and genetic control of anthocyanins in flowers of Actinidia hybrid families segregating for red and white petal colour. Results Four inter-related backcross families between Actinidia chinensis Planch. var. chinensis and Actinidia eriantha Benth. were identified that segregated 1:1 for red or white petal colour. Flower pigments consisted of five known anthocyanins (two delphinidin-based and three cyanidin-based) and three unknowns. Intensity and hue differed in red petals from pale pink to deep magenta, and while intensity of colour increased with total concentration of anthocyanin, no association was found between any particular anthocyanin data and hue. Real time qPCR demonstrated that an R2R3 MYB, MYB110a, was expressed at significant levels in red-petalled progeny, but not in individuals with white petals. A microsatellite marker was developed that identified alleles that segregated with red petal colour, but not with ovary, stamen filament, or fruit flesh colour in these families. The marker mapped to chromosome 10 in Actinidia. The white petal phenotype was complemented by syringing Agrobacterium tumefaciens carrying Actinidia 35S::MYB110a into the petal tissue. Red pigments developed in white petals both with, and without, co-transformation with Actinidia bHLH partners. MYB110a was shown to directly activate Actinidia F3GT1 in transient assays. Conclusions The transcription factor, MYB110a, regulates anthocyanin production in petals in this hybrid population, but not in other flower tissues or mature fruit. The identification of delphinidin-based anthocyanins in these flowers provides candidates for colour enhancement in novel fruits.