Development and characterization of microsatellite loci for two species of Beringian birds, rock sandpiper (Calidris ptilocnemis) and Pacific wren (Troglodytes pacificus)
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- 作者：Christin L. Pruett (1)
Cesili Whelan (1)
Angela Ricono (1)
Stacey L. Lance (2)
Travis Glenn (3)
Brant Faircloth (4)
Kevin Winker (5) - 关键词：Troglodytes ; Calidris ; PCR primers ; Microsatellite ; Aleutian Islands ; Pribilof Islands
- 刊名：Conservation Genetics Resources
- 出版年：2014
- 出版时间：March 2014
- 年：2014
- 卷：6
- 期：1
- 页码：175-177
- 全文大小：156 KB
- 作者单位：Christin L. Pruett (1)
Cesili Whelan (1)
Angela Ricono (1)
Stacey L. Lance (2)
Travis Glenn (3)
Brant Faircloth (4)
Kevin Winker (5)
1. Department of Biological Sciences, Florida Institute of Technology, Melbourne, FL, 32901, USA
2. Savannah River Ecology Laboratory, University of Georgia, Aiken, SC, 29802, USA
3. Department of Environmental Health Science, University of Georgia, Athens, GA, 30602, USA
4. Department of Ecology and Evolutionary Biology, University of California, Los Angeles, CA, 90095, USA
5. University of Alaska Museum, Fairbanks, AK, 99775, USA - ISSN：1877-7260
文摘
Identification and assessment of small, endemic populations are priorities for conservation. We isolated and characterized 8 microsatellite loci from rock sandpiper (Calidris ptilocnemis) and 5 microsatellite loci from Pacific wren (Troglodytes pacificus), species with endemic populations of named subspecies that are of conservation concern. Eighteen to 20 individuals of each species from several locations in Alaska were screened for polymorphism. Loci for each species showed high polymorphism, with rock sandpiper ranging from 5 to 14 alleles per locus and 0.73-.88 expected heterozygosity and Pacific wren ranging from 5 to 14 alleles per locus and 0.55-.91 expected heterozygosity. Loci developed for rock sandpipers were also polymorphic in closely related taxa. These loci are the first developed for either species and will be used to identify and conserve endemic populations in the Bering Sea region.