Effect of Chemistry on Osteogenesis and Angiogenesis Towards Bone Tissue Engineering Using 3D Printed Scaffolds

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• 作者：Susmita Bose ; Solaiman Tarafder ; Amit Bandyopadhyay
• 关键词：Matrix mineralization ; Bone histomorphometry ; 3D printing ; Calcium phosphates ; Osteogenesis ; Angiogenesis ; Injury/fracture healing
• 刊名：Annals of Biomedical Engineering
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：45
• 期：1
• 页码：261-272
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine, general; Biomedical Engineering; Biological and Medical Physics, Biophysics; Classical Mechanics; Biochemistry, general;
• 出版者：Springer US
• ISSN：1573-9686
• 卷排序：45

文摘

The functionality or survival of tissue engineering constructs depends on the adequate vascularization through oxygen transport and metabolic waste removal at the core. This study reports the presence of magnesium and silicon in direct three dimensional printed (3DP) tricalcium phosphate (TCP) scaffolds promotes in vivo osteogenesis and angiogenesis when tested in rat distal femoral defect model. Scaffolds with three different interconnected macro pore sizes were fabricated using direct three dimensional printing. In vitro ion release in phosphate buffer for 30 days showed sustained Mg2+ and Si4+ release from these scaffolds. Histolomorphology and histomorphometric analysis from the histology tissue sections revealed a significantly higher bone formation, between 14 and 20% for 4–16 weeks, and blood vessel formation, between 3 and 6% for 4–12 weeks, due to the presence of magnesium and silicon in TCP scaffolds compared to bare TCP scaffolds. The presence of magnesium in these 3DP TCP scaffolds also caused delayed TRAP activity. These results show that magnesium and silicon incorporated 3DP TCP scaffolds with multiscale porosity have huge potential for bone tissue repair and regeneration.