Highly sensitive electroluminescence immunoassay for Hg(II) ions based on the use of CdSe quantum dots, the methylmercury-6-mercaptonicotinic acid-ovalbumin conjugate, and a specific monoclonal antibody
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- 作者：Jing Zhang ; Mingbo Wang ; Xun Yao ; Anping Deng ; Jianguo Li
- 关键词：Electrochemiluminescence ; Immunosensor ; Mercury(II) ion ; CdSe quantum dots ; Monoclonal antibody
- 刊名：Microchimica Acta
- 出版年：2015
- 出版时间：February 2015
- 年：2015
- 卷：182
- 期：3-4
- 页码：469-477
- 全文大小：952 KB
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文摘
We have designed a rapid and ultrasensitive electrochemiluminescent (ECL) competitive immunoassay for the determination of mercury(II) ion. It is based on the use of CdSe quantum dots (QDs), methylmercury-6-mercaptonicotinic acid-ovalbumin as coating antigen and specific monoclonal antibodies (mAbs) against Hg(II). The latter is quite selective for Hg(II). The coating antigen was immobilized on the surface of a gold electrode via reaction between the functional groups of cysteamine and glutaraldehyde. The mercury(II) ions in a sample and the coating antigen compete for binding sites of QD-labeled monoclonal antibody which binds specifically to Hg(II) ions. The ECL of the system decreases with increasing concentration of Hg(II) because less QD-labeled mAbs are present on the surface of the electrode. Under optimal conditions, the decrease of ECL intensity is linearly related to the logarithm of the Hg(II) concentration in the range from 0.02 to 100?ng?mL?, with a detection limit of 6.2?pg?mL?. As far as we know, this is the first report on an ECL immunoassay for Hg(II) based on a specific monoclonal antibody. The favorable results obtained when this method was applied to real samples indicate that this detection scheme can widely enlarge the applicability of detecting heavy metal ions by exploiting the ECL of QDs for immunoassays. Graphical Abstract A rapid and ultrasensitive electrochemiluminescent competitive immunoassay for the mercury (II) based on CdSe quantum dots is put forward. It has been used in the detection of real samples with satisfactory results, indicating a promising method for trace mercury and other small molecular compounds.