Assembly and features of secondary metabolite biosynthetic gene clusters in Streptomyces ansochromogenes

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• 作者：XingYu Zhong (14506) (24506)
  YuQing Tian (14506)
  GuoQing Niu (14506)
  HuaRong Tan (14506)
  
• 关键词：secondary metabolite ; gene cluster ; assemble ; features ; draft genome
• 刊名：Science China Life Sciences
• 出版年：2013
• 出版时间：July 2013
• 年：2013
• 卷：56
• 期：7
• 页码：609-618
• 全文大小：930KB
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• 作者单位：XingYu Zhong (14506) (24506)
  YuQing Tian (14506)
  GuoQing Niu (14506)
  HuaRong Tan (14506)
  
  14506. State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China
  24506. University of Chinese Academy of Sciences, Beijing, 100049, China
  

文摘

A draft genome sequence of Streptomyces ansochromogenes 7100 was generated using 454 sequencing technology. In combination with local BLAST searches and gap filling techniques, a comprehensive antiSMASH-based method was adopted to assemble the secondary metabolite biosynthetic gene clusters in the draft genome of S. ansochromogenes. A total of at least 35 putative gene clusters were identified and assembled. Transcriptional analysis showed that 20 of the 35 gene clusters were expressed in either or all of the three different media tested, whereas the other 15 gene clusters were silent in all three different media. This study provides a comprehensive method to identify and assemble secondary metabolite biosynthetic gene clusters in draft genomes of Streptomyces, and will significantly promote functional studies of these secondary metabolite biosynthetic gene clusters.