Role of berberine in anti-bacterial as a high-affinity LPS antagonist binding to TLR4/MD-2 receptor

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• 作者：Ming Chu (10)
  Ran Ding (11)
  Zheng-yun Chu (11)
  Ming-bo Zhang (11)
  Xiao-yan Liu (10)
  Shao-hua Xie (10)
  Yan-jun Zhai (11)
  Yue-dan Wang (10)
  
• 刊名：BMC Complementary and Alternative Medicine
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：14
• 期：1
• 全文大小：397 KB
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  54. The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1472-6882/14/89/prepub
  
• 作者单位：Ming Chu (10)
  Ran Ding (11)
  Zheng-yun Chu (11)
  Ming-bo Zhang (11)
  Xiao-yan Liu (10)
  Shao-hua Xie (10)
  Yan-jun Zhai (11)
  Yue-dan Wang (10)
  
  10. Department of Immunology, School of Basic Medical Sciences, Peking University, No.38, Xueyuan Road, Haidian District, Beijing, 100191, China
  11. Pharmacy Departments, Liao Ning University of Traditional Chinese Medicine, Liao Ning, 116600, China
  
• ISSN：1472-6882

文摘

Background Berberine is an isoquinoline alkaloid mainly extracted from Rhizoma Coptidis and has been shown to possess a potent inhibitory activity against bacterial. However, the role of berberine in anti-bacterial action has not been extensively studied. Methods The animal model was established to investigate the effects of berberine on bacterial and LPS infection. Docking analysis, Molecular dynamics simulations and Real-time RT-PCR analysis was adopted to investigate the molecular mechanism. Results Treatment with 40?mg/kg berberine significantly increased the survival rate of mice challenged with Salmonella typhimurium (LT2), but berberine show no effects in bacteriostasis. Further study indicated that treatment with 0.20?g/kg berberine markedly increased the survival rate of mice challenged with 2 EU/ml bacterial endotoxin (LPS) and postpone the death time of the dead mice. Moreover, pretreatment with 0.05?g/kg berberine significantly lower the increasing temperature of rabbits challenged with LPS. The studies of molecular mechanism demonstrated that Berberine was able to bind to the TLR4/MD-2 receptor, and presented higher affinity in comparison with LPS. Furthermore, berberine could significantly suppressed the increasing expression of NF-κB, IL-6, TNFα, and IFNβ in the RAW264.7 challenged with LPS. Conclusion Berberine can act as a LPS antagonist and block the LPS/TLR4 signaling from the sourse, resulting in the anti-bacterial action.