Ultrastructural effects and antibiofilm activity of LFchimera against Burkholderia pseudomallei

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• 作者：Aekkalak Puknun ; Sakawrat Kanthawong…
• 关键词：Antimicrobial peptide ; Biofilm ; Burkholderia pseudomallei ; LFchimera ; (Immune) electron microscopy ; Melioidosis
• 刊名：World Journal of Microbiology & Biotechnology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：32
• 期：2
• 全文大小：3,288 KB
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• 作者单位：Aekkalak Puknun (1) (2) (3) (7)
  Sakawrat Kanthawong (1) (2) (4)
  Chitchanok Anutrakunchai (1) (3) (4) (5)
  Kamran Nazmi (3)
  Wikky Tigchelaar (6)
  Kees A. Hoeben (6)
  Enno C. I. Veerman (3)
  Jan G. M. Bolscher (3)
  Suwimol Taweechaisupapong (1) (4) (8)
  
  1. Biofilm Research Group, Faculty of Dentistry, Khon Kaen University, Khon Kaen, 40002, Thailand
  2. Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand
  3. Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Gustav Mahlerlaan 3004, 1081 LA, Amsterdam, The Netherlands
  7. School of Medical Science, University of Phayao, Mueang Phayao, Phayao, 56000, Thailand
  4. Melioidosis Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand
  5. Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand
  6. Core Facility Cellular Imaging/LCAM-AMC, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands
  8. Department of Oral Diagnosis, Faculty of Dentistry, Khon Kaen University, Khon Kaen, 40002, Thailand
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Applied Microbiology
  Biotechnology
  Biochemistry
  Environmental Biotechnology
  Microbiology
  
• 出版者：Springer Netherlands
• ISSN：1573-0972

文摘

Lactoferrin chimera (LFchimera), a hybrid peptide containing the two antimicrobial stretches of the innate immunity factor bovine lactoferrin, viz. LFampin265-284 and LFcin17-30, has strikingly high antimicrobial activity against the category B pathogen Burkholderia pseudomallei. The action mechanisms of LFchimera against B. pseudomallei is not fully understood. The aim of this study was to further investigate the effect of treated B. pseudomallei with LFchimera using (immune) electron microscopy. The effects of LFchimera on biofilm formation and against preformed biofilm of B. pseudomallei were also determined. After exposure to LFchimera, transmission electron microscopy revealed swelling of the periplasmic space of B. pseudomallei and a highly inhomogeneous electron density in the intracellular DNA region. Localization of LFchimera in B. pseudomallei using immunoelectron microscopy showed gold particles in intracellular structures without accumulation on the membranes. LFchimera also possessed stronger bactericidal activity than ceftazidime against B. pseudomallei grown in biofilm. Moreover, limited exposure of B. pseudomallei to LFchimera at subcidal concentration could reduce biofilm formation. Altogether, the results indicate that LFchimera possesses antibacterial and antibiofilm activities and can modulate B. pseudomallei colonization. Therefore, the efficacy of LFchimera merits further development of this agent for the therapy of melioidosis.