Plant-derived mAbs have effective anti-cancer activities by increasing ganglioside expression in colon cancers

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• 作者：Jae-Sung Ryu (1)
  Ju-Taek Lee (1)
  Malg-Um Lim (1)
  Mi-Ran Hwang (1)
  Kyung-A Hwang (2)
  Young-Ho Cho (3)
  Jeong-Hwan Lee (4)
  Kisung Ko (4)
  Young-Kug Choo (1)
  
• 关键词：Apoptosis ; Colon cancer ; Gangliosides ; Monoclonal antibody ; Plant ; derived mAb CO17 ; 1A
• 刊名：Biotechnology Letters
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：35
• 期：12
• 页码：2031-2038
• 全文大小：
• 作者单位：Jae-Sung Ryu (1)
  Ju-Taek Lee (1)
  Malg-Um Lim (1)
  Mi-Ran Hwang (1)
  Kyung-A Hwang (2)
  Young-Ho Cho (3)
  Jeong-Hwan Lee (4)
  Kisung Ko (4)
  Young-Kug Choo (1)
  
  1. Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan, Jeonbuk, 570-749, Korea
  2. Department of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration, Suwon, 441-853, Korea
  3. Department of Pharmaceutical Engineering, Konyang University, Nonsan, 320-711, Korea
  4. Department of Medicine, Medical Research Institute, College of Medicine, Chung-Ang University, Heukseok-ro 84, Seoul, 156-756, Korea
  
• ISSN：1573-6776

文摘

An epithelial cell adhesion molecule (EpCAM) was selectively expressed in human colorectal carcinoma. Treatment with plant-derived anti-EpCAM mAb (mAbP CO17-1A) and RAW264.7 cells inhibited cell growth in the human colorectal cancer cell line SW620. In SW620 treated with mAbP CO17-1A and RAW264.7 cells, expression of p53 and p21 increased, whereas the expression of G1 phase-related proteins, cyclin D1, CDK4, cyclin E, and CDK2, decreased, similar to mammalian-derived mAb (mAbM) CO17-1A. Similar to mAbM CO17-1A, treatment with mAbP CO17-1A and RAW264.7 cell decreased the expression of anti-apoptotic protein, Bcl-2, but the expression of pro-apoptotic proteins Bax, TNF-α, caspase-3, caspase-6, caspase-8 and caspase-9, increased. Cells treated with mAbP CO17-1A and RAW264.7 cells expressed metastasis-related gangliosides, GM1 and GD1a, similar to mAbM CO17-1A. These results suggest that mAbP CO17-1A is as effective on anti-cancer activity as mAbM CO17-1A.