Functional analysis of monoclonal antibodies against the Plasmodium falciparum PfEMP1-VarO adhesin

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• 作者：Micheline Guillotte ; Farida Nato ; Alexandre Juillerat ; Audrey Hessel…
• 关键词：Malaria ; Rosetting ; PfEMP1 adhesin ; Monoclonal antibodies (mAbs) ; Rosette ; disrupting antibodies ; Epitopes ; Antigenicity
• 刊名：Malaria Journal
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：15
• 期：1
• 全文大小：4,748 KB
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• 作者单位：Micheline Guillotte (1) (2)
  Farida Nato (3)
  Alexandre Juillerat (4) (5)
  Audrey Hessel (1) (4)
  Françoise Marchand (3)
  Anita Lewit-Bentley (4) (5)
  Graham A. Bentley (4) (5)
  Inès Vigan-Womas (1) (2) (6)
  Odile Mercereau-Puijalon (1) (2)
  
  1. Institut Pasteur, Unité d’Immunologie Moléculaire des Parasites, 25-28 rue du Dr ROUX, 75015, Paris, France
  2. URA CNRS 2581, 25-28 rue du Dr ROUX, 75015, Paris, France
  3. Institut Pasteur, Plate-forme de Production de Protéines recombinantes et d’Anticorps (PF5), 25-28 rue du Dr ROUX, 75015, Paris, France
  4. Institut Pasteur, Unité d’Immunologie Structurale, 25-28 rue du Dr ROUX, 75015, Paris, France
  5. CNRS URA 2185, 25-28 rue du Dr ROUX, 75015, Paris, France
  6. Institut Pasteur de Madagascar, Unité d’Immunologie des Maladies Infectieuses, BP 1274, Antananarivo 101, Madagascar
  
• 刊物主题：Parasitology; Infectious Diseases; Tropical Medicine;
• 出版者：BioMed Central
• ISSN：1475-2875

文摘

Background Rosetting, namely the capacity of the Plasmodium falciparum-infected red blood cells to bind uninfected RBCs, is commonly observed in African children with severe malaria. Rosetting results from specific interactions between a subset of variant P. falciparum erythrocyte membrane protein 1 (PfEMP1) adhesins encoded by var genes, serum components and RBC receptors. Rosette formation is a redundant phenotype, as there exists more than one var gene encoding a rosette-mediating PfEMP1 in each genome and hence a diverse array of underlying interactions. Moreover, field diversity creates a large panel of rosetting-associated serotypes and studies with human immune sera indicate that surface-reacting antibodies are essentially variant-specific. To gain better insight into the interactions involved in rosetting and map surface epitopes, a panel of monoclonal antibodies (mAbs) was investigated.