A Study of Anti Beta-2 Glycoprotein I and Anti-Prothrombin Antibodies in Patients with Unexplained Recurrent Pregnancy Losses

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• 作者：Angad Singh ; Anita Nangia ; Sunita Sharma…
• 关键词：Anticardiolipin ; Antiphospholipid syndrome ; Lupus coagulation inhibitor ; Pregnancy ; Prothrombin ; Beta 2 ; glycoprotein I
• 刊名：Indian Journal of Hematology and Blood Transfusion
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：32
• 期：2
• 页码：198-201
• 全文大小：386 KB
• 参考文献：1.Whittaker PG, Taylor A, Lind T (1983) Unsuspected pregnancy loss in healthy women. Lancet 1:1126–1127CrossRef PubMed
  2.Wilcox AJ, Weinberg CR, O’Connor JF, Baird DD, Schlatterer JP, Canfield RE et al (1988) Incidence of early loss in pregnancy. N Eng J Med 319:189–194CrossRef
  3.Lissalde-Lavigne G, Cochery-Nouvellon E, Mercier E, Quéré I, Dauzat M, Marès P, Gris JC (2005) The association between hereditary thrombophilia and pregnancy loss. Haematologica 90:1223–1230PubMed
  4.Kumar KS, Jyothy A, Prakash, Rani HS, Reddy PP (2002) Beta2-glycoprotein I dependent anticardiolipin antibodies and lupus anticoagulant in patients with recurrent pregnancy loss. J Postgrad Med 48:5–10PubMed
  5.Miyakis S, Lockshin MD, Atsumi T, Branch DW, Brey RL, Cervera R et al (2006) International consensus statement on an update of the classification criteria for definite antiphospholipid syndrome (APS). J Thromb Haemost 4:295–306CrossRef PubMed
  6.Galli M, Comfurius P, Maassen C, Hemker HC et al (1990) Anticardiolipin antibodies (ACA) directed not to cardiolipin but to a plasma protein cofactor. Lancet 335:1544–1547CrossRef PubMed
  7.McNeil HP, Simpson RJ, Chesterman CN, Krilis SA (1990) Anti-phospholipid antibodies are directed against a complex antigen that includes a lipid-binding inhibitor of coagulation: beta 2-glycoprotein I (apolipoprotein H). Proc Natl Acad Sci USA 87:4120–4124CrossRef PubMed PubMedCentral
  8.Matsuura E, Igarashi Y, Fujimoto M, Ichikawa K, Koike T (1990) Anticardiolipin cofactor (s) and differential diagnosis of autoimmune disease. Lancet 336:177–178CrossRef PubMed
  9.Philip De Groot (2012) Urbanus Rolf T. The significance of autoantibodies against beta 2 glycoprotein I. Blood 120(2):266–274CrossRef
  10.Bevers EM, Galli M, Barbui T, Comfurius P, Zwaal RF (1991) Lupus anticoagulant IgGs (LA) are not directed to phospholipids only, but to a complex of lipid-bound human prothrombin. Thromb Haemost 66:629–632PubMed
  11.Fleck RA, Rapaport SI, Rao LV (1988) Anti-prothrombin antibodies and the lupus anticoagulant. Blood 72:512–519PubMed
  12.Permpikul P, Rao LV, Rapaport SI (1994) Functional and binding studies of the roles of prothrombin and beta 2 glycoprotein I in the expression of lupus anticoagulant activity. Blood 83:2878–2892PubMed
  13.Galli M, Finazzi G, Bevers EM, Barbui T (1995) Kaolin clotting time and dilute Russell’s viper venom time distinguish between prothrombin-dependent and beta 2-glycoprotein I-dependent antiphospholipid antibodies. Blood 86:617–623PubMed
  14.Vora S, Shetty S, Salvi V, Satoskar P, Ghosh K (2008) A comprehensive screening analysis of antiphospholipid antibodies in Indian women with fetal loss. Eur J Rep Biol 137(2):136–140
  15.Bizzaro N, Tonutti E, Villalta D, Tampoia M, Tozzoli R (2005) Prevalence and clinical correlation of anti-phospholipid–binding protein antibodies in anticardiolipin-negative patients with systemic lupus erythematosus and women with unexplained recurrent miscarriages. Arch Pathol Lab Med 129:61–68PubMed
  16.Zammiti W, Mtiraoui N, Kallel C, Mercier E, Almawi WY, Mahjoub T (2006) A case-control study on the association of idiopathic recurrent pregnancy loss with autoantibodies against b2-glycoprotein I and annexin V. Reproduction 131:817–822CrossRef PubMed
  17.Sater MS, Finan RR, Abu-Hijleh FM, Abu-Hijleh TM, Almawi WY (2012) Anti-phosphatidylserine, anti-cardiolipin, anti-β2 glycoprotein I and anti-prothrombin antibodies in recurrent miscarriage at 8–12 gestational weeks. Eur J Obstet Gynecol Reprod Biol 163(2):170–174CrossRef PubMed
  18.Marozio L, Curti A, Botta G, Canuto EM, Salton L, Tavella AM, Benedetto C (2011) Antiprothrombin antibodies are associated with adverse pregnancy outcome. Am J Reprod Immunol 66:404–409CrossRef PubMed
  19.Conti F, Capozzi A, Truglia S, Lococo E, Longo A, Misasi R et al (2014) The mosaic of ‘‘Seronegative’’ antiphospholipid syndrome. J Immunol Res. doi:10.​1155/​2014/​389601
  20.Andreoli L, Nalli C, Borghi MO, Pregnolato F, Grossi C, Zangola A et al (2013) Domain I is the main specificity of anti-beta2 GPI in systemic autoimmune diseases. Arthritis Rheum 65:S4CrossRef
  
• 作者单位：Angad Singh (1)
  Anita Nangia (1)
  Sunita Sharma (1)
  Manju Puri (2)
  
  1. Department of Pathology, Lady Hardinge Medical College and Associated Hospitals, New Delhi, 110001, India
  2. Department of Obstetrics & Gynaecology, Lady Hardinge Medical College and Associated Hospitals, New Delhi, 110001, India
  
• 刊物主题：Hematology; Oncology; Blood Transfusion Medicine; Human Genetics;
• 出版者：Springer India
• ISSN：0974-0449

文摘

To compare the levels of IgG and IgM anti beta-2 glycoprotein I antibodies and IgG and IgM anti prothrombin antibodies among women with unexplained recurrent pregnancy losses and women with at least 2 live issues. To compare the prevalence of newer anti beta-2 glycoprotein I & anti prothrombin antibodies with conventional Lupus anticoagulant & anticardiolipin antibodies. 50 women with recurrent pregnancy losses & 50 matched controls were evaluated for the presence of: Lupus anticoagulant—screened by LA sensitive aPTT& DRVV and confirmatory Staclot Assay. ELISA kits were used for detecting IgG & IgM anticardiolipin, anti beta-2 glycoprotein I & anti prothrombin antibodies. 11/50 (22 %) women in study group and none in control group had circulating antiphospholipid antibodies. 2 cases (4 %) had lupus anticoagulant. 1 case (2 %) had anticardiolipin antibody & 6 cases (12 %) were positive for anti beta-2 Glycoprotein I antibody (p value = 0.027). 3 cases (6 %) had anti prothrombin antibody. All were mutually exclusive except for one. Women with recurrent pregnancy losses should be tested for anti beta-2 Glycoprotein I antibodies & anti prothrombin antibodies in addition to conventional lupus anticoagulant and anticardiolipin antibodies. This approach can decrease the incidence of SNAP (seronegative antiphospholipid syndrome) cases while establishing the true prevalence of antiphospholipid syndrome.