Polymerase Chain Reaction molecular diagnostic technology for monitoring chronic osteomyelitis

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• 作者：Brian D Mariani ; Daniel S Martin ; Antonia F Chen…
• 关键词：Polymerase chain reaction ; Osteomyelitis ; Molecular diagnosis ; Bacterial infection ; Staphylococcus aureus
• 刊名：Journal of Experimental Orthopaedics
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：1
• 期：1
• 全文大小：755KB
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• 作者单位：Brian D Mariani (1) (3)
  Daniel S Martin (1) (4)
  Antonia F Chen (2)
  Haruyo Yagi (2)
  Sheldon S Lin (1) (5)
  Rocky S Tuan (1) (2)
  
  1. Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA, USA
  3. Molecular Infectious Disease Laboratory, Genetics & IVF Institute, 3015 Williams Drive, Fairfax, VA, 22031, USA
  4. Prime Health Network, 9聽N Brookside Road, Springfield, PA, 19064, USA
  2. Center for Cellular and Molecular Engineering, Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, 450 Technology Drive, Pittsburgh, PA, 15219, USA
  5. Department of Orthopaedic Surgery, Rutgers-New Jersey Medical School, 90 Bergen Street Room 1200, Newark, NJ, 07101, USA
  
• 刊物类别：Orthopedics;
• 刊物主题：Orthopedics;
• 出版者：Springer Berlin Heidelberg
• ISSN：2197-1153

文摘

Background Osteomyelitis is a devastating condition whose treatment relies on the detection of bacteria. The current standard of microbiology culture may not be adequate. Molecular biology based diagnostic procedures for detecting bacteria in orthopaedic infections was previously established, but has not been applied to the setting of chronic osteomyelitis. We aim to determine the applicability of molecular diagnostic procedures for monitoring chronic osteomyelitis, and to evaluate if these procedures are superior to standard culture methods of osteomyelitis detection.