Proteomic analysis of Pteropus alecto kidney cells in response to the viral mimic, Poly I:C
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- 作者：Lawrence Mok ; James W. Wynne ; Kris Ford ; Brian Shiell ; Antony Bacic…
- 关键词：Quantitative proteomics ; DIGE ; iTRAQ ; Bat cells ; Poly I ; C ; Viral mimic ; dsRNA analogue
- 刊名：Proteome Science
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：13
- 期：1
- 全文大小：1,631 KB
- 刊物主题：Proteomics;
- 出版者：BioMed Central
- ISSN：1477-5956
文摘
Background Bats are recognised as an important reservoir for a number of highly pathogenic zoonotic viruses. While many of these viruses cause severe and often fatal disease in humans, bats are able to coexist with these viruses without clinical signs of disease. The mechanism conferring this antiviral response is not fully understood. Here, we investigated the differential protein expression of immortalised Pteropus alecto kidney cells (PaKiT03) following transfection with the viral mimic, Poly I:C. Two complementary proteomic approaches, difference gel electrophoresis (DIGE) and isobaric tagging for relative and absolute quantitation (iTRAQ) were used to quantify changes in protein expression following Poly I:C stimulation at 4, 8 and 20 hr post treatment (hpt).