Identification of a novel group of putative Arabidopsis thaliana β-(1,3)-galactosyltransferases

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• 作者：Yongmei Qu ; Jack Egelund ; Paul R. Gilson ; Fiona Houghton ; Paul A. Gleeson ; Carolyn J. Schultz and Antony Bacic
• 关键词：Arabinogalactan ; protein ; CAZy ; database ; Galactosyltransferase ; Glycosyltransferase ; Golgi
• 刊名：Plant Molecular Biology
• 出版年：2008
• 出版时间：September, 2008
• 年：2008
• 卷：68
• 期：1-2
• 页码：43-59
• 全文大小：1.5 MB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Biochemistry
  Plant Pathology
  
• 出版者：Springer Netherlands
• ISSN：1573-5028

文摘

To begin biochemical and molecular studies on the biosynthesis of the type II arabinogalactan chains on arabinogalactan-proteins (AGPs), we adopted a bioinformatic approach to identify and systematically characterise the putative galactosyltransferases (GalTs) responsible for synthesizing the β-(1,3)-Gal linkage from CAZy GT-family-31 from Arabidopsis thaliana. These analyses confirmed that 20 members of the GT-31 family contained domains/motifs typical of biochemically characterised β-(1,3)-GTs from mammalian systems. Microarray data confirm that members of this family are expressed throughout all tissues making them likely candidates for the assembly of the ubiquitously found AGPs. One member, At1g77810, was selected for further analysis including location studies that confirmed its presence in the Golgi and preliminary enzyme substrate specificity studies that demonstrated β-(1,3)-GalT activity. This bioinformatic/molecular study of CAZy GT-family-31 was validated by the recent report of Strasser et al. (Plant Cell 19:2278–2292, 2007) that another member of this family (At1g26810; GALT1) encodes a β-(1,3)-GalT involved in the biosynthesis of the Lewis a epitope of N-glycans in Arabidopsis thaliana.