Chrysanthemum low-temperature storage and cryopreservation: a review

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• 作者：Jaime A. Teixeira da Silva ; Haenghoon Kim…
• 关键词：Cold storage ; Cryostorage ; Encapsulation ; In vitro storage ; Liquid nitrogen ; Plant growth regulators
• 刊名：Plant Cell, Tissue and Organ Culture
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：120
• 期：2
• 页码：423-440
• 全文大小：387 KB
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• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1573-5044

文摘

Chrysanthemum (Dendranthema?×?grandiflora (Ramat.) Kitamura) is an ornamental plant that responds well to in vitro growth conditions. This receptivity makes it a particularly attractive target for low-temperature storage and cryopreservation studies. This review examines in detail the protocols thus far used to achieve the short- to long-term low-temperature and cryostorage of important chrysanthemum germplasm. Occasionally, medicinal chrysanthemum species have also been cryostored, and these studies are also examined in detail. Since chrysanthemum is sensitive to both osmotic stress and chemical toxicity of vitrification solutions, a generalized protocol for the cryopreservation of chrysanthemum apical or axillary shoot tips is proposed: excision of apical or axillary shoot tips after 4 or 7?weeks, respectively, from final subculture; progressive preculture with 10?% sucrose for 31?h, 17.5?% sucrose for 17?h, then 25?% sucrose for 7?h; osmoprotection with 17.5?% glycerol?+?17.5?% sucrose for 40?min; cryoprotection with PVS3 (50?% glycerol?+?50?% sucrose) vitrification solution for 60?min (axillary) or 90?min (apical); cooling and warming using aluminium foil strips; unloading with 30?% sucrose for 40?min. When smaller axillary shoot tips are used, cryoprotection of samples with 37.5?% glycerol?+?15?% DMSO?+?15?% ethylene glycol?+?22.5?% sucrose at 0?°C for about 60?min can be applied.