The impact of breed and tissue compartment on the response of pig macrophages to lipopolysaccharide

详细信息    查看全文

• 作者：Ronan Kapetanovic (1)
  Lynsey Fairbairn (2)
  Alison Downing (1)
  Dario Beraldi (3)
  David P Sester (4)
  Tom C Freeman (1)
  Christopher K Tuggle (5)
  Alan L Archibald (1)
  David A Hume (1)
  
• 关键词：Pig ; Macrophages ; Microarray ; Breed ; Lipopolysaccharide
• 刊名：BMC Genomics
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：14
• 期：1
• 全文大小：2144KB
• 参考文献：1. Hume DA: The mononuclear phagocyte system. / Curr Opin Immunol 2006,18(1):49鈥?3. CrossRef
  2. Weidenbusch M, Anders HJ: Tissue microenvironments define and get reinforced by macrophage phenotypes in homeostasis or during inflammation, repair and fibrosis. / J Innate Immun 2012,4(5鈥?):463鈥?77. CrossRef
  3. Nilsson R, Bajic VB, Suzuki H, di Bernardo D, Bjorkegren J, Katayama S, Reid JF, Sweet MJ, Gariboldi M, Carninci P, / et al.: Transcriptional network dynamics in macrophage activation. / Genomics 2006,88(2):133鈥?42. CrossRef
  4. Litvak V, Ramsey SA, Rust AG, Zak DE, Kennedy KA, Lampano AE, Nykter M, Shmulevich I, Aderem A: Function of C/EBPdelta in a regulatory circuit that discriminates between transient and persistent TLR4-induced signals. / Nat Immunol 2009,10(4):437鈥?43. CrossRef
  5. Schroder K, Irvine KM, Taylor MS, Bokil NJ, Le Cao KA, Masterman KA, Labzin LI, Semple CA, Kapetanovic R, Fairbairn L, / et al.: Conservation and divergence in toll-like receptor 4-regulated gene expression in primary human versus mouse macrophages. / Proc Natl Acad Sci U S A 2012,109(16):E944-E953. CrossRef
  6. Roshick C, Wood H, Caldwell HD, McClarty G: Comparison of gamma interferon-mediated antichlamydial defense mechanisms in human and mouse cells. / Infect Immun 2006,74(1):225鈥?38. CrossRef
  7. Thoma-Uszynski S, Stenger S, Takeuchi O, Ochoa MT, Engele M, Sieling PA, Barnes PF, Rollinghoff M, Bolcskei PL, Wagner M, / et al.: Induction of direct antimicrobial activity through mammalian toll-like receptors. / Science 2001,291(5508):1544鈥?547. CrossRef
  8. Seok J, Warren HS, Cuenca AG, Mindrinos MN, Baker HV, Xu W, Richards DR, McDonald-Smith GP, Gao H, Hennessy L, / et al.: Genomic responses in mouse models poorly mimic human inflammatory diseases. / Proc Natl Acad Sci U S A 2013,110(9):3507鈥?512. CrossRef
  9. Dawson HD, Loveland JE, Pascal G, Gilbert JG, Uenishi H, Mann KM, Sang Y, Zhang J, Carvalho-Silva D, Hunt T, / et al.: Structural and functional annotation of the porcine immunome. / BMC Genomics 2013, 14:332. CrossRef
  10. Fairbairn L, Kapetanovic R, Sester DP, Hume DA: The mononuclear phagocyte system of the pig as a model for understanding human innate immunity and disease. / J Leukoc Biol 2011,89(6):855鈥?1. CrossRef
  11. Ait-Ali T, Wilson AD, Westcott DG, Clapperton M, Waterfall M, Mellencamp MA, Drew TW, Bishop SC, Archibald AL: Innate immune responses to replication of porcine reproductive and respiratory syndrome virus in isolated swine alveolar macrophages. / Viral Immunol 2007,20(1):105鈥?18. CrossRef
  12. Moser RJ, Reverter A, Lehnert SA: Gene expression profiling of porcine peripheral blood leukocytes after infection with actinobacillus pleuropneumoniae. / Vet Immunol Immunopathol 2008,121(3鈥?):260鈥?74. CrossRef
  13. Ait-Ali T, Wilson AD, Carre W, Westcott DG, Frossard JP, Mellencamp MA, Mouzaki D, Matika O, Waddington D, Drew TW, / et al.: Host inhibits replication of European porcine reproductive and respiratory syndrome virus in macrophages by altering differential regulation of type-I interferon transcriptional response. / Immunogenetics 2011,63(7):437鈥?48. CrossRef
  14. Groenen MA, Archibald AL, Uenishi H, Tuggle CK, Takeuchi Y, Rothschild MF, Rogel-Gaillard C, Park C, Milan D, Megens HJ, / et al.: Analyses of pig genomes provide insight into porcine demography and evolution. / Nature 2012,491(7424):393鈥?98. CrossRef
  15. Freeman TC, Ivens A, Baillie JK, Beraldi D, Barnett MW, Dorward D, Downing A, Fairbairn L, Kapetanovic R, Raza S, / et al.: A gene expression atlas of the domestic pig. / BMC biology 2012, 10:90. CrossRef
  16. Kapetanovic R, Fairbairn L, Beraldi D, Sester DP, Archibald AL, Tuggle CK, Hume DA: Pig bone marrow-derived macrophages resemble human macrophages in their response to bacterial lipopolysaccharide. / J Immunol 2012,188(7):3382鈥?394. CrossRef
  17. Fairbairn L, Kapetanovic R, Beraldi D, Sester DP, Tuggle CK, Archibald AL, Hume DA: Comparative analysis of monocyte subsets in the pig. / J Immunol 2013,190(12):6389鈥?396. CrossRef
  18. Gow DJ, Sester DP, Hume DA: CSF-1, IGF-1, and the control of postnatal growth and development. / J Leukoc Biol 2010,88(3):475鈥?81. CrossRef
  19. Morozumi T, Uenishi H: Polymorphism distribution and structural conservation in RNA-sensing toll-like receptors 3, 7, and 8 in pigs. / Biochim Biophys Acta 2009,1790(4):267鈥?74. CrossRef
  20. Bergman IM, Rosengren JK, Edman K, Edfors I: European wild boars and domestic pigs display different polymorphic patterns in the toll-like receptor (TLR) 1, TLR2, and TLR6 genes. / Immunogenetics 2010,62(1):49鈥?8. CrossRef
  21. Kruithof EK, Satta N, Liu JW, Dunoyer-Geindre S, Fish RJ: Gene conversion limits divergence of mammalian TLR1 and TLR6. / BMC Evol Biol 2007, 7:148. CrossRef
  22. Takeuchi O, Kawai T, Muhlradt PF, Morr M, Radolf JD, Zychlinsky A, Takeda K, Akira S: Discrimination of bacterial lipoproteins by toll-like receptor 6. / Int Immunol 2001,13(7):933鈥?40. CrossRef
  23. Trapnell BC, Whitsett JA: Gm-CSF regulates pulmonary surfactant homeostasis and alveolar macrophage-mediated innate host defense. / Annual review of physiology 2002, 64:775鈥?02. CrossRef
  24. Hsing CH, Chiu CJ, Chang LY, Hsu CC, Chang MS: IL-19 is involved in the pathogenesis of endotoxic shock. / Shock 2008,29(1):7鈥?5.
  25. Kusza S, Flori L, Gao Y, Teillaud A, Hu R, Lemonnier G, Bosze Z, Bourneuf E, Vincent-Naulleau S, Rogel-Gaillard C: Transcription specificity of the class Ib genes SLA-6, SLA-7 and SLA-8 of the swine major histocompatibility complex and comparison with class Ia genes. / Anim Genet 2011,42(5):510鈥?20. CrossRef
  26. da Huang W, Sherman BT, Lempicki RA: Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. / Nat Protoc 2009,4(1):44鈥?7. CrossRef
  27. MacDonald KP, Palmer JS, Cronau S, Seppanen E, Olver S, Raffelt NC, Kuns R, Pettit AR, Clouston A, Wainwright B, / et al.: An antibody against the colony-stimulating factor 1 receptor depletes the resident subset of monocytes and tissue- and tumor-associated macrophages but does not inhibit inflammation. / Blood 2010,116(19):3955鈥?963. CrossRef
  28. Shibata Y, Zsengeller Z, Otake K, Palaniyar N, Trapnell BC: Alveolar macrophage deficiency in osteopetrotic mice deficient in macrophage colony-stimulating factor is spontaneously corrected with age and associated with matrix metalloproteinase expression and emphysema. / Blood 2001,98(9):2845鈥?852. CrossRef
  29. Niida S, Kondo T, Hiratsuka S, Hayashi S, Amizuka N, Noda T, Ikeda K, Shibuya M: VEGF receptor 1 signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient mice. / Proc Natl Acad Sci U S A 2005,102(39):14016鈥?4021. CrossRef
  30. Fejer G, Wegner MD, Gyory I, Cohen I, Engelhard P, Voronov E, Manke T, Ruzsics Z, Dolken L, Prazeres da Costa O, / et al.: Nontransformed, GM-CSF-dependent macrophage lines are a unique model to study tissue macrophage functions. / Proc Natl Acad Sci U S A 2013,110(24):E2191鈥?198. CrossRef
  31. Endale Ahanda ML, Fritz ER, Estelle J, Hu ZL, Madsen O, Groenen MA, Beraldi D, Kapetanovic R, Hume DA, Rowland RR, / et al.: Prediction of altered 3'- UTR miRNA-binding sites from RNA-Seq data: the swine leukocyte antigen complex (SLA) as a model region. / PLoS One 2012,7(11):e48607. CrossRef
  32. Kojima-Shibata C, Shinkai H, Morozumi T, Jozaki K, Toki D, Matsumoto T, Kadowaki H, Suzuki E, Uenishi H: Differences in distribution of single nucleotide polymorphisms among intracellular pattern recognition receptors in pigs. / Immunogenetics 2009,61(2):153鈥?60. CrossRef
  33. Theocharidis A, van Dongen S, Enright AJ, Freeman TC: Network visualization and analysis of gene expression data using BioLayout express(3D). / Nat Protoc 2009,4(10):1535鈥?550. CrossRef
  
• 作者单位：Ronan Kapetanovic (1)
  Lynsey Fairbairn (2)
  Alison Downing (1)
  Dario Beraldi (3)
  David P Sester (4)
  Tom C Freeman (1)
  Christopher K Tuggle (5)
  Alan L Archibald (1)
  David A Hume (1)
  
  1. The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, Edinburgh, EH25 9RG, United Kingdom
  2. Institute for Medical Microbiology, Immunology and Hygiene, Technische Universit盲t M眉nchen, 81679, Munich, Germany
  3. Cancer Research UK Cambridge Research Institute, Li Ka Shing Centre, Robinson Way, Cambridge, CB2 0RE, UK
  4. Innate Immunity Laboratory, School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland, QLD 4072, Australia
  5. Department of Animal Science, Iowa State University, Ames, IA, 50011, USA
  

文摘

Background The draft genome of the domestic pig (Sus scrofa) has recently been published permitting refined analysis of the transcriptome. Pig breeds have been reported to differ in their resistance to infectious disease. In this study we examine whether there are corresponding differences in gene expression in innate immune cells Results We demonstrate that macrophages can be harvested from three different compartments of the pig (lungs, blood and bone-marrow), cryopreserved and subsequently recovered and differentiated in CSF-1. We have performed surface marker analysis and gene expression profiling on macrophages from these compartments, comparing twenty-five animals from five different breeds and their response to lipopolysaccharide. The results provide a clear distinction between alveolar macrophages (AM) and monocyte-derived (MDM) and bone-marrow-derived macrophages (BMDM). In particular, the lung macrophages express the growth factor, FLT1 and its ligand, VEGFA at high levels, suggesting a distinct pathway of growth regulation. Relatively few genes showed breed-specific differential expression, notably CXCR2 and CD302 in alveolar macrophages. In contrast, there was substantial inter-individual variation between pigs within breeds, mostly affecting genes annotated as being involved in immune responses. Conclusions Pig macrophages more closely resemble human, than mouse, in their set of macrophage-expressed and LPS-inducible genes. Future research will address whether inter-individual variation in macrophage gene expression is heritable, and might form the basis for selective breeding for disease resistance.