Melatonin modulates the expression of BCL-xl and improve the development of vitrified embryos obtained by IVF in mice

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• 作者：Maryam Dehghani-Mohammadabadi (1)
  Mohammad Salehi (2) (3)
  Fattaneh Farifteh (2)
  Sedigheh Nematollahi (1)
  Ehsan Arefian (4)
  Atena Hajjarizadeh (4)
  Kazem Parivar (5)
  Zahra Nourmohammadi (5)
  
• 关键词：Melatonin ; Vitrification ; IVF ; Reactive oxygen species ; Apoptosis
• 刊名：Journal of Assisted Reproduction and Genetics
• 出版年：2014
• 出版时间：April 2014
• 年：2014
• 卷：31
• 期：4
• 页码：453-461
• 全文大小：675 KB
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• 作者单位：Maryam Dehghani-Mohammadabadi (1)
  Mohammad Salehi (2) (3)
  Fattaneh Farifteh (2)
  Sedigheh Nematollahi (1)
  Ehsan Arefian (4)
  Atena Hajjarizadeh (4)
  Kazem Parivar (5)
  Zahra Nourmohammadi (5)
  
  1. Department of Transgenic Animal Science, Stem Cell Technology Research Center, Tehran, Iran
  2. Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, 193954717, Tehran, Iran
  3. Department of Biotechnology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  4. Department of Molecular Biology and Genetic Engineering, Stem Cell Technology Research Center, Tehran, Iran
  5. Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
  
• ISSN：1573-7330

文摘

Purpose Antioxidant and anti-apoptotic effects of melatonin on development of in vitro fertilization (IVF)/vitrified two-cell mouse embryos were evaluated in this study. Methods The IVF two-cell embryos were vitrified by cryotop, and were cultured in KSOM medium in different concentrations of melatonin (10?, 10?, 10?2?M) and without melatonin. The blastocyst cell number, apoptotic cells and glutathione (GSH) level were evaluated by differential, TUNEL and cell tracker blue staining, respectively. The expression of Bax and Bcl-xl genes was evaluated by qPCR. The expression of melatonin receptors (Mtnr1a and Mtnr1b) in mouse 2-cell embryos and blastocysts was evaluated by RT-PCR. Results Melatonin increased the rate of cleavage and blastulation at 10?2?M concentration (p-lt;-.05). The number of trophectoderm and inner cell mass showed a significant increase (p-lt;-.05) in 10??M melatonin. The 10??M and 10?2?M melatonin treatments significantly reduced (p-lt;-.05) the apoptotic index. The significant increase in the expression of Bcl-xl observed at 10??M concentration however, reduced expression of Bax was not statistically significant. The levels of GSH in 10? and 10?2?M groups were significantly improved relative to the control group (p-lt;-.05). The Mtnr1a was expressed in 2-cell embryos and blastocysts in all groups, but the expression of Mntr1b was not detected. Conclusion Melatonin may have a special role against oxidative stress in protection of IVF/vitrified embryos.