A comparison of RNA-seq and exon arrays for whole genome transcription profiling of the L5 spinal nerve transection model of neuropathic pain in the rat
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- 作者：James R Perkins ; Ana Antunes-Martins ; Margarita Calvo ; John Grist…
- 关键词：Whole ; genome transcription profiling ; Exon arrays ; Microarrays ; RNA ; Sequencing ; RNA ; seq ; Next generation sequencing ; Spinal nerve transection ; Nerve injury ; Neuropathic pain ; Differential gene expression
- 刊名：Molecular Pain
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：10
- 期：1
- 全文大小：4,080 KB
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- 出版者：BioMed Central
- ISSN：1744-8069
文摘
Background The past decade has seen an abundance of transcriptional profiling studies of preclinical models of persistent pain, predominantly employing microarray technology. In this study we directly compare exon microarrays to RNA-seq and investigate the ability of both platforms to detect differentially expressed genes following nerve injury using the L5 spinal nerve transection model of neuropathic pain. We also investigate the effects of increasing RNA-seq sequencing depth. Finally we take advantage of the “agnostic-approach of RNA-seq to discover areas of expression outside of annotated exons that show marked changes in expression following nerve injury. Results RNA-seq and microarrays largely agree in terms of the genes called as differentially expressed. However, RNA-seq is able to interrogate a much larger proportion of the genome. It can also detect a greater number of differentially expressed genes than microarrays, across a wider range of fold changes and is able to assign a larger range of expression values to the genes it measures. The number of differentially expressed genes detected increases with sequencing depth. RNA-seq also allows the discovery of a number of genes displaying unusual and interesting patterns of non-exonic expression following nerve injury, an effect that cannot be detected using microarrays. Conclusion We recommend the use of RNA-seq for future high-throughput transcriptomic experiments in pain studies. RNA-seq allowed the identification of a larger number of putative candidate pain genes than microarrays and can also detect a wider range of expression values in a neuropathic pain model. In addition, RNA-seq can interrogate the whole genome regardless of prior annotations, being able to detect transcription from areas of the genome not currently annotated as exons. Some of these areas are differentially expressed following nerve injury, and may represent novel genes or isoforms. We also recommend the use of a high sequencing depth in order to detect differential expression for genes with low levels of expression.