EventPointer: an effective identification of alternative splicing events using junction arrays
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- 作者：Juan P. Romero ; Ander Muniategui ; Fernando J. De Miguel ; Ander Aramburu…
- 关键词：KeywordAlternative splicing ; Junction microarrays ; Protein domains
- 刊名：BMC Genomics
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：17
- 期：1
- 全文大小：2294KB
- 刊物主题：Life Sciences, general; Microarrays; Proteomics; Animal Genetics and Genomics; Microbial Genetics and Genomics; Plant Genetics & Genomics;
- 出版者：BioMed Central
- ISSN：1471-2164
- 卷排序：17
文摘
BackgroundAlternative splicing (AS) is a major source of variability in the transcriptome of eukaryotes. There is an increasing interest in its role in different pathologies. Before sequencing technology appeared, AS was measured with specific arrays. However, these arrays did not perform well in the detection of AS events and provided very large false discovery rates (FDR). Recently the Human Transcriptome Array 2.0 (HTA 2.0) has been deployed. It includes junction probes. However, the interpretation software provided by its vendor (TAC 3.0) does not fully exploit its potential (does not study jointly the exons and junctions involved in a splicing event) and can only be applied to case–control studies. New statistical algorithms and software must be developed in order to exploit the HTA 2.0 array for event detection.