An alternative analysis of enzyme systems based on the whole reaction time: evaluation of the kinetic parameters and initial enzyme concentration

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• 作者：R. Var¨®n ; M. Garcia-Moreno ; J. Masi¨¢-P¨¦rez ; F. Garc¨ªa-Molina ; F. Garc¨ªa-C¨¢novas ; E. Arias ; E. Arribas and F. Garc¨ªa-Sevilla
• 关键词：Enzyme kinetics ; Michaelis– ; Menten ; integrated equation ; product rate ; substrate rate ; numerical integration
• 刊名：Journal of Mathematical Chemistry
• 出版年：2007
• 出版时间：November, 2007
• 年：2007
• 卷：42
• 期：4
• 页码：789-813
• 全文大小：434 KB

文摘

This work presents an alternative analysis of the integrated rate equations corresponding to the simple Michaelis-Menten mechanism without product inhibition. The suggested new results are reached under a minimal set of assumptions and include, as a particular case, the classical integrated Michaelis–Menten equation. Experimental designs and a kinetic data analysis are suggested to the estimation of the maximum steady-state rate, V _max, the Michaelis–Menten constant, K _m, the initial enzyme concentration, [E]₀, and the catalytic constant, k ₂. The goodness of the analysis is tested with simulated time progress curves obtained by numerical integration.