Genetic analysis of arsenic metabolism in Micrococcus luteus BPB1, isolated from the Bengal basin
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- 作者：Vrajan Vijay ; Kozhikode Bhagavathiparambu Vandana…
- 关键词：Arsenic ; Soil bacteria ; Arsenic resistance ; Arsenate reductase ; Arsenite transporter ; Biosorption
- 刊名：Annals of Microbiology
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：67
- 期：1
- 页码：79-89
- 全文大小：
- 刊物主题：Microbiology; Microbial Genetics and Genomics; Microbial Ecology; Mycology; Medical Microbiology; Applied Microbiology;
- 出版者：Springer Berlin Heidelberg
- ISSN：1869-2044
- 卷排序：67
文摘
A highly arsenic-metabolizing bacterial strain was isolated from an agricultural field known for arsenic contamination near Munshiganj (Bangladesh). Based on 16S rRNA gene analysis, the strain was identified as Micrococcus luteus and designated as strain BPB1. Arsenate and arsenite minimal inhibitory concentrations of 650 mM and 7.5 mM, respectively, were observed for strain BPB1, slightly higher than the figures observed in its close relative M. luteus DSM 20030T. Such observations were consistent with the presence of arsenic-metabolizing genes in the genome of M. luteus. We describe this strain as having an MSH/Mrx-dependent class of arsenate reductase, and an arsenite transporter family in the ACR3(1) group. Besides an intracellular arsenic resistance mechanism, experiments carried out using field emission scanning electron microscopy-energy dispersive X-ray spectroscopy (FESEM-EDS) and Fourier transform infrared spectroscopy (FTIR) demonstrated the ability of BPB1 to sequester arsenate in extracellular polymeric substances on its cell surface.