Identification of Putative Artemisia annua ABCG Transporter Unigenes Related to Artemisinin Yield Following Expression Analysis in Different Plant Tissues and in Response to Methyl Jasmonate and Abscisic Acid Treatments

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• 作者：Ling Zhang (1)
  Xu Lu (1)
  Qian Shen (1)
  Yunfei Chen (1)
  Tao Wang (1)
  Fangyuan Zhang (1)
  Shaoyan Wu (1)
  Weimin Jiang (1)
  Pin Liu (1)
  Lida Zhang (1)
  Yueyue Wang (1)
  Kexuan Tang (1) kxtang1@yahoo.com
• 关键词：ABCG transporter &#8211 ; Artemisia annua &#8211 ; Artemisinin &#8211 ; ADS &#8211 ; CYP71AV1 &#8211 ; Trichome cuticle
• 刊名：Plant Molecular Biology Reporter
• 出版年：2012
• 出版时间：August 2012
• 年：2012
• 卷：30
• 期：4
• 页码：838-847
• 全文大小：614.6 KB
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• 作者单位：1. Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240 China
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1572-9818

文摘

Artemisinin has attracted interest due to its medicinal value in treating malaria and its potential for use against certain cancers and viral diseases. Trichome density and capacity determine artemisinin content in Artemisia annua plants. Thus, the ATP-binding cassette transporter G (ABCG) subfamily involved in trichome cuticle development may also influence artemisinin accumulation. In this study, putative A. annua ABC transporter unigenes were identified and classified from the unigene sequences up to date in the National Center for Biotechnology Information database, and nine putative A. annua ABCG transporter unigenes that may be involved in cuticle development were selected for expression analyses. Two of them, AaABCG6 and AaABCG7, showed parallel expression pattern as two artemisinin biosynthesis-specific genes (amorpha-4, 11-diene synthase and a cytochrome P450-dependent hydroxylase, CYP71AV1) in different tissues and different leaf development stages and also showed similar induction in the plants after methyl jasmonate or abscisic acid treatments. Identification of these putative A. annua ABCG transporter unigenes could provide the basis for cloning of the full-length genes and further functional investigation to find the artemisinin relevant transporters, which could be used for improving artemisinin yield in both A. annua plants and heterologous systems using transgenic technology.