Generation of a cre recombinase-conditional Nos1ap over-expression transgenic mouse

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• 作者：Dallas R. Auer (1)
  Polina Sysa-Shah (2)
  Djahida Bedja (2)
  Jessica L. Simmers (1)
  Evgenia Pak (3)
  Amalia Dutra (3)
  Ronald Cohn (4)
  Kathleen L. Gabrielson (2)
  Aravinda Chakravarti (1)
  Ashish Kapoor (1)
  
• 关键词：Cardiac arrhythmia ; Conditional over ; expression ; GENOME ; wide association studies ; NOS1AP ; QT interval ; Sudden cardiac death ; Transgenic mouse
• 刊名：Biotechnology Letters
• 出版年：2014
• 出版时间：June 2014
• 年：2014
• 卷：36
• 期：6
• 页码：1179-1185
• 全文大小：
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• 作者单位：Dallas R. Auer (1)
  Polina Sysa-Shah (2)
  Djahida Bedja (2)
  Jessica L. Simmers (1)
  Evgenia Pak (3)
  Amalia Dutra (3)
  Ronald Cohn (4)
  Kathleen L. Gabrielson (2)
  Aravinda Chakravarti (1)
  Ashish Kapoor (1)
  
  1. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, 733聽N. Broadway, Baltimore, MD, 21205, USA
  2. Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, 733聽N. Broadway, Baltimore, MD, 21205, USA
  3. National Human Genome Research Institute, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD, 20892, USA
  4. The Hospital for Sick Children, University of Toronto, 555 University Avenue, Toronto, ON, M5G 1X8, Canada
  
• ISSN：1573-6776

文摘

Polymorphic non-coding variants at the NOS1AP locus have been associated with the common cardiac, metabolic and neurological traits and diseases. Although, in vitro gene targeting-based cellular and biochemical studies have shed some light on NOS1AP function in cardiac and neuronal tissue, to enhance our understanding of NOS1AP function in mammalian physiology and disease, we report the generation of cre recombinase-conditional Nos1ap over-expression transgenic mice (Nos1ap Tg). Conditional transgenic mice were generated by the pronuclear injection method and three independent, single-site, multiple copies integration event-based founder lines were selected. For heart-restricted over-expression, Nos1ap Tg mice were crossed with Mlc2v-cre and Nos1ap transcript over-expression was observed in left ventricles from Nos1ap Tg; Mlc2v-cre F1 mice. We believe that with the potential of conditional over-expression, Nos1ap Tg mice will be a useful resource in studying NOS1AP function in various tissues under physiological and disease states.