Determination of protein, fat, starch, and amino acids in foxtail millet [Setaria italica (L.) Beauv.] by Fourier transform near-infrared reflectance spectroscopy

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• 作者：Xiu-Shi Yang (1) (2)
  Li-Li Wang (2) (3)
  Xian-Rong Zhou (2)
  Shao-Min Shuang (3)
  Zhi-Hua Zhu (2)
  Nan Li (2)
  Yan Li (2)
  Fang Liu (2)
  San-Cai Liu (2)
  Ping Lu (2)
  Gui-Xing Ren (2)
  Chuan Dong (1)
  
• 关键词：foxtail millet ; protein ; starch ; amino acid ; NIRS
• 刊名：Food Science and Biotechnology
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：22
• 期：6
• 页码：1495-1500
• 全文大小：199 KB
• 作者单位：Xiu-Shi Yang (1) (2)
  Li-Li Wang (2) (3)
  Xian-Rong Zhou (2)
  Shao-Min Shuang (3)
  Zhi-Hua Zhu (2)
  Nan Li (2)
  Yan Li (2)
  Fang Liu (2)
  San-Cai Liu (2)
  Ping Lu (2)
  Gui-Xing Ren (2)
  Chuan Dong (1)
  
  1. Research Center of Environmental Science and Engineering, Shanxi University, Taiyuan, 030006, China
  2. Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China
  3. School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan, 030006, China
  
• ISSN：2092-6456

文摘

Quantitative detection of protein, fat, starch, and amino acids in foxtail millet using Fourier transform near-infrared spectroscopy (NIRS) was investigated. Foxtail millet samples (n=259) were analyzed using NIRS. Spectral data were linearized with data from chemical analyses. Calibration models were established using a partial least-squares (PLS) algorithm with cross-validation. Optimized models were tested using external validation set samples with coefficients of determination in the external validation (R 2 val) of >0.90. Residual predictive deviation (RPD) values were nearly equal to or >2.5 for crude protein, alanine, aspartic acid, glutamic acid, isoleucine, leucine, and serine. However, for glycine, histidine, phenylalanine, proline, threonine, tyrosine, and valine, the R 2 val values were >0.83 and RPD values were nearly equal to or >2.0. For crude fat, total starch, arginine, and lysine, the R 2 val values were >0.70 and RPD values were >1.5. NIRS is a rapid determination tool for foxtail millet breeding, and for quality control.