Impacts of environmental stress on growth, secondary metabolite biosynthetic gene clusters and metabolite production of xerotolerant/xerophilic fungi
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- 作者：Angel Medina ; Markus Schmidt-Heydt ; Alicia Rodríguez ; Roberto Parra…
- 关键词：Water ; Temperature ; Stress ; Mycotoxins ; Gene clusters ; Gene function ; Systems biology ; Modelling
- 刊名：Current Genetics
- 出版年：2015
- 出版时间：August 2015
- 年：2015
- 卷：61
- 期：3
- 页码：325-334
- 全文大小：1,059 KB
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Marín P, Magan N, Vázquez C, González-Jaén MT (2010b) Differential effect of environmental conditions on growth and regulation of the fumonisin biosynthetic gene FUM1 in the maize pathogens and fumonisin-producers F. verticillioides and F. proliferatum. FEMS Microbiol Ecol 73:303-11PubMed
Medina A, Schmidt-Heydt M, Cárdenas-Chávez DL, Parra R, Geisen R, Magan N (2013) Integrating toxin gene expression, growth and fumonisin B1 and B2 production by a strain of Fusarium vertic - 作者单位：Angel Medina (1)
Markus Schmidt-Heydt (2)
Alicia Rodríguez (1)
Roberto Parra (3)
Rolf Geisen (2)
Naresh Magan (1)
1. Applied Mycology Group, Cranfield Soil and AgriFood Institute, Cranfield University, Cranfield, Bedford, MK43 0AL, UK
2. Max Rubner Institute, Karlsruhe, Germany
3. Biotechnology Centre, Monterrey Technology, Monterrey, Mexico - 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Microbial Genetics and Genomics
Microbiology
Biochemistry
Cell Biology
Plant Sciences
Proteomics - 出版者：Springer Berlin / Heidelberg
- ISSN：1432-0983
文摘
This paper examines the impact that single and interacting environmental stress factors have on tolerance mechanisms, molecular ecology and the relationship with secondary metabolite production by a group of mycotoxigenic species of economic importance. Growth of these fungi (Aspergillus flavus, A.ochraceus, A.carbonarius, Penicillium nordicum and P. verrucosum) is influenced by water and temperature interactions and type of solute used to induce water stress. Such abiotic stresses are overcome by the synthesis of increased amounts of low molecular weight sugar alcohols, especially glycerol and erythritol, to enable them to remain active under abiotic stress. This is accompanied by increased expression of sugar transporter genes, e.g., in A. flavus, which provides the nutritional means of tolerating such stress. The optimum conditions of water activity (a w)?×?temperature stress for growth are often different from those for secondary metabolite production. The genes for toxin production are clustered together and their relative expression is influenced by abiotic interacting stress factors. For example., A. flavus synthesises aflatoxins under water stress in non-ionic solutes. In contrast, P. nordicum specifically occupies a high salt (0.87 a w?=?22?% NaCl) niche such as cured meats, and produces ochratoxin A (OTA). There is differential and temporal expression of the genes in the secondary metabolite clusters in response to a w?×?temperature stress. We have used a microarray and integrated data on growth, relative expression of key genes in the biosynthetic pathways for secondary metabolite production and toxin production using a mixed growth model. This was used to correlate these factors and predict the toxin levels produced under different abiotic stress conditions. This system approach to integrate these different data sets and model the relationships could be a powerful tool for predicting the relative toxin production under extreme stress conditions, including climate change scenarios. This approach will facilitate a better functional understanding of the influence that environmental stress has on these mycotoxigenic fungi and enable better prevention strategies to be developed based on this system-based approach.