Differentiating tomato leaf curl viruses possessing mono/bi partite genomes using replicase gene based PCR assay: implications for developing virus specific resistance

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• 作者：Varsha Garg ; Vipin Permar ; Vilas Parkhi…
• 关键词：Monopartite ; Bipartite ; Ty based resistance loci
• 刊名：Journal of Plant Biochemistry and Biotechnology
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：24
• 期：4
• 页码：461-465
• 全文大小：1,173 KB
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• 作者单位：Varsha Garg (1)
  Vipin Permar (1)
  Vilas Parkhi (2)
  Elangovan Mani (2)
  Abdulrahman A. Alatar (3)
  Mohammad Faisal (3)
  Shelly Praveen (1)
  
  1. Advanced Center for Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110012, India
  2. Advanta Ltd., Krishnama House, Road No.7, Banjara Hills, Hyderabad, 500034, India
  3. Department of Botany & Microbiology, College of Science, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia
  
• 刊物主题：Life Sciences, general; Plant Biochemistry; Protein Science; Receptors; Cell Biology;
• 出版者：Springer India
• ISSN：0974-1275

文摘

Tomato leaf curl disease (ToLCD) is associated with begomoviruses, with their generic names as Tomato leaf curl viruses (ToLCVs) and Tomato yellow leaf curl viruses (TYLCVs). ToLCVs exist both with mono/bipartite genomes, whereas TYLCVs carry monopartite genomes. For management of these viruses, three resistance loci Ty-1 to Ty-3 are being deployed, which provides different degree of resistance. These viruses encode a replication initiator protein (Rep), indispensible for virus replication. The conserved/divergent sequences of rep gene(s) were used to develop mono/bipartite virus specific PCR assay. The implications of distinguishing viruses with mono/bipartite genomes for using virus specific resistance loci through breeding have been discussed. Keywords Monopartite Bipartite Ty based resistance loci