Genotoxicity Assessment of Volatile Organic Compounds in Landfill Gas Emission Using Comet Assay in Higher Terrestrial Plant

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• 作者：Veerapas Na roi-et ; Wilai Chiemchaisri…
• 关键词：BTEX ; Comet assay ; Genotoxicity ; Plant ; Landfill gas ; VOCs
• 刊名：Bulletin of Environmental Contamination and Toxicology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：98
• 期：2
• 页码：283-289
• 全文大小：
• 刊物主题：Pollution, general; Environmental Health; Ecotoxicology; Soil Science & Conservation; Environmental Chemistry; Waste Water Technology / Water Pollution Control / Water Management / Aquatic Pollution;
• 出版者：Springer US
• ISSN：1432-0800
• 卷排序：98

文摘

Genotoxicity model is developed to assess the individual subacute toxicity of benzene, toluene, ethylbenzene, and xylene (BTEX) at very low levels as in a landfill gas. Golden Pothos (Epipremnum aureum), a higher plant, was tested under variation of benzene 54–5656 ng/L, toluene 10–4362 ng/L, ethylbenzene 28–4997 ng/L, xylene 53–4845 ng/L, for 96 h. DNA fragmentation in plant leaves were investigated via comet assay. The results show that DNA migration ratio increased with the BTEX concentrations, but at different rates. The 50% effective concentration (EC₅₀) of DNA fragmentation from the dose–response relationships indicated toluene has the highest EC₅₀ value and followed by benzene, xylene and ethylbenzene. Alternatively, ethylbenzene has the highest toxicity unit and followed by xylene, benzene and toluene as described by toxicity unit (TU). In conclusion, comet assay of Pothos can be used in differentiating DNA fragmentation against very low levels of BTEX in the atmosphere. Pothos is recommended for genotoxicity assessment of a low BTEX contaminated atmosphere.