Identification and expression analysis of IRAK-4 cDNA and its gene from the ayu Plecoglossus altivelis altivelis

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• 作者：Kyuma Suzuki ; Shotaro Izumi ; Hideki Tanaka ; Takayuki Katagiri
• 关键词：Ayu Plecoglossus altivelis altivelis ; Innate and adaptive immunities ; Interleukin ; 1 receptor ; associated kinase 4 (IRAK ; 4) ; mRNA expression analysis ; Flavobacterium psychrophilum
• 刊名：Fisheries Science
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：82
• 期：1
• 页码：47-57
• 全文大小：690 KB
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• 作者单位：Kyuma Suzuki (1)
  Shotaro Izumi (2)
  Hideki Tanaka (1)
  Takayuki Katagiri (3)
  
  1. Gunma Prefectural Fisheries Experimental Station, Shikishima 13, Maebashi, Gunma, 371-0036, Japan
  2. Course of Applied Biological Science, Department of Fisheries, School of Marine Science and Technology, Tokai University, Orido, Shimizu, Shizuoka, 424-8610, Japan
  3. Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato, Tokyo, 108-8477, Japan
  
• 刊物主题：Fish & Wildlife Biology & Management; Freshwater & Marine Ecology; Food Science;
• 出版者：Springer Japan
• ISSN：1444-2906

文摘

Interleukin-1 receptor-associated kinase 4 (IRAK-4) is an important component of the mammalian innate and adaptive defense system against infectious disease. In this study, ayu Plecoglossus altivelis altivelis IRAK-4 cDNA and genomic DNA were cloned and analyzed. The open reading frame of ayu IRAK-4 cDNA encodes 463 amino acids, including a death domain and a serine/threonine protein kinase domain of the mammalian IRAK-4 motif. The ayu IRAK-4 gene comprises 11 exons, and was found to be very similar to vertebrate IRAK-4 genes described thus far. Ayu IRAK-4 mRNA was expressed in all tissues, and predominantly in the gill, heart, intestine, whole kidney, and spleen. At 12 h after immersion challenge with Flavobacterium psychrophilum, ayu IRAK-4 mRNA expression in the whole blood was up-regulated (21.6-fold) compared to the control. The present study suggests that ayu IRAK-4 may be involved in the immune response. Keywords Ayu Plecoglossus altivelis altivelis Innate and adaptive immunities Interleukin-1 receptor-associated kinase 4 (IRAK-4) mRNA expression analysis Flavobacterium psychrophilum