Construction of transplastomic lettuce (Lactuca sativa) dominantly producing astaxanthin fatty acid esters and detailed chemical analysis of generated carotenoids
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- 作者：Hisashi Harada (1) (2) (5)
Takashi Maoka (1) (3)
Ayako Osawa (4)
Jun-ichiro Hattan (1)
Hirosuke Kanamoto (1) (2)
Kazutoshi Shindo (4)
Toshihiko Otomatsu (2)
Norihiko Misawa (1) - 关键词：Astaxanthin ; Carotenoid ; Lettuce ; Lactuca sativa ; Chloroplast transformation ; Pathway engineering
- 刊名：Transgenic Research
- 出版年：2014
- 出版时间：April 2014
- 年：2014
- 卷：23
- 期：2
- 页码：303-315
- 全文大小：849 KB
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Takashi Maoka (1) (3)
Ayako Osawa (4)
Jun-ichiro Hattan (1)
Hirosuke Kanamoto (1) (2)
Kazutoshi Shindo (4)
Toshihiko Otomatsu (2)
Norihiko Misawa (1)
1. Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, 1-308 Suematsu, Nonoichi-shi, Ishikawa, 921-8836, Japan
2. KNC Bio Research Center, KNC Laboratories Co., Ltd., 1-1-1 Murodani, Nishi-ku, Kobe, 651-2241, Japan
5. Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, 4-101 Koyamacho-minami, Tottori-shi, Tottori, 680-8552, Japan
3. Research Institute for Production Development, 15 Shimogamo-morimoto-cho, Sakyo-ku, Kyoto, 606-0805, Japan
4. Department of Food and Nutrition, Japan Women’s University, 2-8-1 Mejirodai, Bunkyo-ku, Tokyo, 112-8681, Japan - ISSN：1573-9368
文摘
The plastid genome of lettuce (Lactuca sativa L.) cv. Berkeley was site-specifically modified with the addition of three transgenes, which encoded β,β-carotenoid 3,3-hydroxylase (CrtZ) and β,β-carotenoid 4,4-ketolase (4,4-oxygenase; CrtW) from a marine bacterium Brevundimonas sp. strain SD212, and isopentenyl diphosphate isomerase from a marine bacterium Paracoccus sp. strain N81106. Constructed transplastomic lettuce plants were able to grow on soil at a growth rate similar to that of non-transformed lettuce cv. Berkeley and generate flowers and seeds. The germination ratio of the lettuce transformants (T0) (98.8?%) was higher than that of non-transformed lettuce (93.1?%). The transplastomic lettuce (T1) leaves produced the astaxanthin fatty acid (myristate or palmitate) diester (49.2?% of total carotenoids), astaxanthin monoester (18.2?%), and the free forms of astaxanthin (10.0?%) and the other ketocarotenoids (17.5?%), which indicated that artificial ketocarotenoids corresponded to 94.9?% of total carotenoids (230?μg/g fresh weight). Native carotenoids were there lactucaxanthin (3.8?%) and lutein (1.3?%) only. This is the first report to structurally identify the astaxanthin esters biosynthesized in transgenic or transplastomic plants producing astaxanthin. The singlet oxygen-quenching activity of the total carotenoids extracted from the transplastomic leaves was similar to that of astaxanthin (mostly esterified) from the green algae Haematococcus pluvialis.