Astragaloside IV possesses antiarthritic effect by preventing interleukin 1β-induced joint inflammation and cartilage damage

详细信息    查看全文

• 作者：Bin Wang (1)
  Min-Zhu Chen (2)
  
• 关键词：Astragaloside IV ; Adjuvant ; induced arthritis ; Macrophage ; Cartilage ; Interleukin 1β
• 刊名：Archives of Pharmacal Research
• 出版年：2014
• 出版时间：June 2014
• 年：2014
• 卷：37
• 期：6
• 页码：793-802
• 全文大小：
• 参考文献：1. Albina, J.E., J.A. Abate, and W.L. Henry Jr. 1991. Nitric oxide production is required for murine resident peritoneal macrophages to suppress mitogen-stimulated T cell proliferation. Role of IFN-gamma in the induction of the nitric oxide-synthesizing pathway. / Journal of Immunology 147: 144-48.
  2. Bao, F., P. Wu, N. Xiao, F. Qiu, and Q.P. Zeng. 2012. Nitric oxide-driven hypoxia initiates synovial angiogenesis, hyperplasia and inflammatory lesions in mice. / PLoS One 7: e34494. CrossRef
  3. Bendele, A. 2001. Animal models of rheumatoid arthritis. / Journal of Musculoskeletal and Neuronal Interactions 1: 377-85.
  4. Chen, D.Y., Y.M. Chen, H.H. Chen, C.W. Hsieh, C.C. Lin, and J.L. Lan. 2011. Increasing levels of circulating Th17 cells and interleukin-17 in rheumatoid arthritis patients with an inadequate response to anti-TNF-alpha therapy. / Arthritis Research & Therapy 13: R126. CrossRef
  5. Cho, W.C., and K.N. Leung. 2007. In vitro and in vivo immunomodulating and immunorestorative effects of Astragalus membranaceus. / Journal of Ethnopharmacology 113: 132-41. CrossRef
  6. Davignon, J.L., M. Hayder, M. Baron, J.F. Boyer, A. Constantin, F. Apparailly, R. Poupot, and A. Cantagrel. 2013. Targeting monocytes/macrophages in the treatment of rheumatoid arthritis. / Rheumatology (Oxford) 52: 590-98. CrossRef
  7. Deknuydt, F., G. Bioley, D. Valmori, and M. Ayyoub. 2009. IL-1beta and IL-2 convert human Treg into T(H)17 cells. / Clinical Immunology 131: 298-07. CrossRef
  8. Deng, S., Y. Xi, H. Wang, J. Hao, X. Niu, W. Li, Y. Tao, and G. Chen. 2010. Regulatory effect of vasoactive intestinal peptide on the balance of Treg and Th17 in collagen-induced arthritis. / Cellular Immunology 265: 105-10. CrossRef
  9. Evans, H.G., N.J. Gullick, S. Kelly, C. Pitzalis, G.M. Lord, B.W. Kirkham, and L.S. Taams. 2009. In vivo activated monocytes from the site of inflammation in humans specifically promote Th17 responses. / Proceedings of the National Academy of Sciences of the United States of America 106: 6232-237. CrossRef
  10. Hu, W., L.J. Xia, F.H. Chen, F.R. Wu, J. Tang, C.Z. Chen, S. Jiang, and H.H. Chen. 2012. Recombinant human endostatin inhibits adjuvant arthritis by down-regulating VEGF expression and suppression of TNF-alpha, IL-1beta production. / Inflammation Research 61: 827-35. CrossRef
  11. Jiang, J.B., J.D. Qiu, L.H. Yang, J.P. He, G.W. Smith, and H.Q. Li. 2010. Therapeutic effects of astragalus polysaccharides on inflammation and synovial apoptosis in rats with adjuvant-induced arthritis. / International Journal of Rheumatic Diseases 13: 396-05. CrossRef
  12. Jouzeau, J.Y., C. Cipolletta, N. Presle, P. Netter, and B. Terlain. 1999. Modulation of IL-1 effects on cartilage by NO synthase inhibitors: pharmacological studies in rats. / Osteoarthritis Cartilage 7: 382-85. CrossRef
  13. Jovanovic, D.V., L. Boumsell, A. Bensussan, X. Chevalier, A. Mancini, and J.A. Di Battista. 2011. CD101 expression and function in normal and rheumatoid arthritis-affected human T cells and monocytes/macrophages. / Journal of Rheumatology 38: 419-28. CrossRef
  14. Ju, J.H., Y.J. Heo, M.L. Cho, J.Y. Jhun, J.S. Park, S.Y. Lee, H.J. Oh, S.J. Moon, S.K. Kwok, K.S. Park, S.H. Park, and H.Y. Kim. 2012. Modulation of STAT-3 in rheumatoid synovial T cells suppresses Th17 differentiation and increases the proportion of Treg cells. / Arthritis and Rheumatism 64: 3543-552. CrossRef
  15. Kinne, R.W., R. Brauer, B. Stuhlmuller, E. Palombo-Kinne, and G.R. Burmester. 2000. Macrophages in rheumatoid arthritis. / Arthritis Research 2: 189-02. CrossRef
  16. Lei, H., B. Wang, W.P. Li, Y. Yang, A.W. Zhou, and M.Z. Chen. 2003. Anti-aging effect of astragalosides and its mechanism of action. / Acta Pharmacologica Sinica 24: 230-34.
  17. Li, H.Q., J.D. Qiu, and L.H. Yang. 2009. Regulation of Astragalus heteropolysaccharides on synoviocytes apoptosis and proinflammatory cytokine secretion of rats with adjuvant arthritis. / Yao Xue Xue Bao 44: 731-36.
  18. Liu, Q.Y., Y.M. Yao, S.W. Zhang, and Z.Y. Sheng. 2011. Astragalus polysaccharides regulate T cell-mediated immunity via CD11c(high)CD45RB(low) DCs in vitro. / Journal of Ethnopharmacology 136: 457-64. CrossRef
  19. Ma, X.Q., Q. Shi, J.A. Duan, T.T. Dong, and K.W. Tsim. 2002. Chemical analysis of Radix Astragali (Huangqi) in China: a comparison with its adulterants and seasonal variations. / Journal of Agriculture and Food Chemistry 50: 4861-866. CrossRef
  20. Nagy, G., A. Koncz, T. Telarico, D. Fernandez, B. Ersek, E. Buzas, and A. Perl. 2010. Central role of nitric oxide in the pathogenesis of rheumatoid arthritis and systemic lupus erythematosus. / Arthritis Research & Therapy 12: 210. CrossRef
  21. Nistala, K., and L.R. Wedderburn. 2009. Th17 and regulatory T cells: rebalancing pro- and anti-inflammatory forces in autoimmune arthritis. / Rheumatology (Oxford) 48: 602-06. CrossRef
  22. Palmer, G., D. Talabot-Ayer, I. Szalay-Quinodoz, M. Maret, W.P. Arend, and C. Gabay. 2003. Mice transgenic for intracellular interleukin-1 receptor antagonist type 1 are protected from collagen-induced arthritis. / European Journal of Immunology 33: 434-40. CrossRef
  23. Prati, C., A. Berthelot, B. Kantelip, D. Wendling, and C. Demougeot. 2012. Treatment with the arginase inhibitor Nw-hydroxy-nor-l -arginine restores endothelial function in rat adjuvant-induced arthritis. / Arthritis Research & Therapy 14: R130.
  24. Presle, N., C. Cipolletta, J.Y. Jouzeau, A. Abid, P. Netter, and B. Terlain. 1999. Cartilage protection by nitric oxide synthase inhibitors after intraarticular injection of interleukin-1beta in rats. / Arthritis and Rheumatism 42: 2094-102. CrossRef
  25. Schett, G., L.C. Coates, Z.R. Ash, S. Finzel, S. Finzel, and P.G. Conaghan. 2011. Structural damage in rheumatoid arthritis, psoriatic arthritis, and ankylosing spondylitis: traditional views, novel insights gained from TNF blockade, and concepts for the future. / Arthritis Research & Therapy 13(Suppl 1): S4.
  26. Stefanovic-Racic, M., K. Meyers, C. Meschter, J.W. Coffey, R.A. Hoffman, and C.H. Evans. 1994. / N-monomethyl arginine, an inhibitor of nitric oxide synthase, suppresses the development of adjuvant arthritis in rats. / Arthritis and Rheumatism 37: 1062-069. CrossRef
  27. Stefanovic-Racic, M., M.O. Mollers, L.A. Miller, and C.H. Evans. 1997. Nitric oxide and proteoglycan turnover in rabbit articular cartilage. / Journal of Orthopaedic Research 15: 442-49. CrossRef
  28. Takayanagi, H. 2007. Osteoimmunology: shared mechanisms and crosstalk between the immune and bone systems. / Nature Reviews Immunology 7: 292-04. CrossRef
  29. Thirion, S., and F. Berenbaum. 2004. Culture and phenotyping of chondrocytes in primary culture. / Methods in Molecular Medicine 100: 1-4.
  30. Van Bezooijen, R.L., L. Van Der Wee-Pals, S.E. Papapoulos, and C.W. Lowik. 2002. Interleukin 17 synergises with tumour necrosis factor alpha to induce cartilage destruction in vitro. / Annals of the Rheumatic Diseases 61: 870-76. CrossRef
  31. Walter, G.J., H.G. Evans, B. Menon, N.J. Gullick, B.W. Kirkham, A.P. Cope, F. Geissmann, and L.S. Taams. 2013. Interaction with activated monocytes enhances cytokine expression and suppressive activity of human CD4?+?CD45ro?+?CD25?+?CD127(low) regulatory T cells. / Arthritis and Rheumatism 65: 627-38. CrossRef
  32. Wang, B., and M.Z. Chen. 1998. Effects of indomethacin on secretory function of synoviocytes from adjuvant arthritis rats. / International Journal of Tissue Reactions 20: 91-4.
  33. Wang, B., L. Ma, X. Tao, and P.E. Lipsky. 2004. Triptolide, an active component of the Chinese herbal remedy / Tripterygium wilfordii Hook F, inhibits production of nitric oxide by decreasing inducible nitric oxide synthase gene transcription. / Arthritis and Rheumatism 50: 303-995.
  34. Wang, B., Y.Y. Yao, and M.Z. Chen. 1998. Effects of indomethacin on joint damage in rat and rabbit. / Zhongguo Yao Li Xue Bao 19: 70-3.
  35. Wang, Y.P., X.Y. Li, C.Q. Song, and Z.B. Hu. 2002. Effect of astragaloside IV on T, B lymphocyte proliferation and peritoneal macrophage function in mice. / Acta Pharmacologica Sinica 23: 263-66.
  36. Yang, L.H., J.D. Qiu, and H.Q. Li. 2009. Effects of Astragalus heteropolysaccharides on erythrocyte immune adherence function of mice with adjuvant-induced arthritis. / Yao Xue Xue Bao 44: 1364-370.
  37. Yao, R., Y. Fu, S. Li, L. Tu, X. Zeng, and N. Kuang. 2011. Regulatory effect of daphnetin, a coumarin extracted from / Daphne odora, on the balance of Treg and Th17 in collagen-induced arthritis. / European Journal of Pharmacology 670: 286-94. CrossRef
  38. Yu, Q.T., L.W. Qi, P. Li, L. Yi, J. Zhao, and Z. Bi. 2007. Determination of seventeen main flavonoids and saponins in the medicinal plant Huang-qi (Radix astragali) by HPLC-DAD-ELSD. / Journal of Separation Science 30: 1292-299. CrossRef
  39. Zivojinovic, S.M., N.N. Pejnovic, M.N. Sefik-Bukilica, L.V. Kovacevic, Ii Soldatovic, and N.S. Damjanov. 2012. Tumor necrosis factor blockade differentially affects innate inflammatory and Th17 cytokines in rheumatoid arthritis. / Journal of Rheumatology 39: 18-1. CrossRef
  
• 作者单位：Bin Wang (1)
  Min-Zhu Chen (2)
  
  1. Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, W-1303 Biomedical Science Tower, 200 Lothrop Street, Pittsburgh, PA, 15261, USA
  2. Institute of Clinical Pharmacology, Anhui Medical University, 81 Meishan Road, Hefei, 230032, Anhui, People’s Republic of China
  
• ISSN：1976-3786

文摘

The saponin astragaloside IV (AST) is one of major active components purified from Astragalus membranaceus (Fisch) Bge, which has been used in traditional Chinese medicine to treat immune disorders including rheumatoid arthritis (RA). The effects of AST on the suppression of experimental arthritis and its possible mechanisms are unknown. We measured the paw swelling of ankle joints, splenocyte proliferation, interleukin 1β (IL-1β), tumor necrosis factor α (TNFα) and nitric oxide (NO) formation by macrophages in rat adjuvant-induced arthritis (AIA). Intraarticular injection of IL-1β to rat knee joint for inducing the edema and in vitro IL-1β-stimulated cartilage impairment were examined. The results showed that oral treatment of AST (100?mg/kg/day) suppressed the joint inflammation and inhibited IL-1β, TNFα and NO production in macrophages from AIA rats. Macrophages were one of AST targeted cells, and mediated the reduced splenocyte proliferation in AIA rats. In addition, AST reduced the swelling induced by intraarticular injection of IL-1β, and protected against IL-1β-induced damage of cartilage proteoglycan synthesis and chondrocyte proliferation. We conclude that AST possesses antiarthritic effect and prevents IL-1β-induced joint inflammation and cartilage destruction. These findings suggest that AST may be used for the treatment of RA and other inflammatory joint diseases.