Preservation of Biomarkers from Cyanobacteria Mixed with MarsLike Regolith Under Simulated Martian Atmosphere and UV Flux
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- 作者：Mickael Baqué ; Cyprien Verseux ; Ute Böttger…
- 关键词：Astrobiology ; Cyanobacteria ; Biosignatures ; Martian regoliths ; EXPOSE ; R2
- 刊名：Origins of Life and Evolution of Biospheres
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：46
- 期：2-3
- 页码：289-310
- 全文大小：5,239 KB
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Cyprien Verseux (1)
Ute Böttger (2)
Elke Rabbow (3)
Jean-Pierre Paul de Vera (2)
Daniela Billi (1) (4)
1. Department of Biology, University of Rome Tor Vergata, Rome, Italy
2. German Aerospace Center (DLR), Institute of Planetary Research, Berlin, Germany
3. Radiation Biology Department, German Aerospace Center (DLR), Institute of Aerospace Medicine, Köln, Germany
4. Dipartimento di Biologia, Università di Roma “Tor Vergata”, Rome, Italy - 刊物主题：Life Sciences, general; Astrophysics and Astroparticles; Earth Sciences, general; Astronomy, Observations and Techniques; Biochemistry, general;
- 出版者：Springer Netherlands
- ISSN：1573-0875
文摘
The space mission EXPOSE-R2 launched on the 24th of July 2014 to the International Space Station is carrying the BIOMEX (BIOlogy and Mars EXperiment) experiment aimed at investigating the endurance of extremophiles and stability of biomolecules under space and Mars-like conditions. In order to prepare the analyses of the returned samples, ground-based simulations were carried out in Planetary and Space Simulation facilities. During the ground-based simulations, Chroococcidiopsis cells mixed with two Martian mineral analogues (phyllosilicatic and sulfatic Mars regolith simulants) were exposed to a Martian simulated atmosphere combined or not with UV irradiation corresponding to the dose received during a 1-year-exposure in low Earth orbit (or half a Martian year on Mars). Cell survival and preservation of potential biomarkers such as photosynthetic and photoprotective pigments or DNA were assessed by colony forming ability assays, confocal laser scanning microscopy, Raman spectroscopy and PCR-based assays. DNA and photoprotective pigments (carotenoids) were detectable after simulations of the space mission (570 MJ/m2 of UV 200–400 nm irradiation and Martian simulated atmosphere), even though signals were attenuated by the treatment. The fluorescence signal from photosynthetic pigments was differently preserved after UV irradiation, depending on the thickness of the samples. UV irradiation caused a high background fluorescence of the Martian mineral analogues, as revealed by Raman spectroscopy. Further investigation will be needed to ensure unambiguous identification and operations of future Mars missions. However, a 3-month exposure to a Martian simulated atmosphere showed no significant damaging effect on the tested cyanobacterial biosignatures, pointing out the relevance of the latter for future investigations after the EXPOSE-R2 mission. Data gathered during the ground-based simulations will contribute to interpret results from space experiments and guide our search for life on Mars.