Detection, partial purification and characterization of bacteriocin produced by Lactobacillus brevis FPTLB3 isolated from freshwater fish

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• 作者：Shiba Prosad Banerjee (1) r> Krushna Chandra Dora (1) r> Supratim Chowdhury (1) r>
• 关键词：Biopreservation ; Lactic acid bacteria ; Lactobacillus brevis ; Bacteriocin ; Antimicrobial activity
• 刊名：Journal of Food Science and Technology
• 出版年：2013
• 出版时间：February 2013
• 年：2013
• 卷：50
• 期：1
• 页码：17-25
• 全文大小：1181KB
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• 作者单位：Shiba Prosad Banerjee (1) r> Krushna Chandra Dora (1) r> Supratim Chowdhury (1) r>r>1. Department of Fish Processing Technology, Faculty of Fishery Sciences, W.B.U.A.F.S, P.O.- Panchasayar, Kolkata, 700 094, India r>
• ISSN：0975-8402

文摘

Lactobacillus brevis FPTLB3 was isolated from freshwater fish, capable of producing bacteriocin that had broad spectrum of inhibition (3200聽AU/ml) against Escherichia coli MTCC 1563, Enterococcus faecalis MTCC 2729, Lactobacillus casei MTCC 1423, Lactobacillus sakei ATCC 15521 and Staphylococcus aureus ATCC 25923. The antimicrobial activity of crude supernatant fluid was stable after heating at 121聽掳C for 60聽min and declined thereafter. Stability of antimicrobial activity was observed at pH range of 2.0 to 8.0. Its active principle was proteinaceous in nature since the bacteriocin was inactivated by proteolytic enzymes, but not by other non鈥損roteolytic enzymes. Mitomycin C and UV light did not affect the activity of the bacteriocin, while chloroform extraction completely destroyed their activity. Exposure to surfactant resulted in an increase in titre, except Nonidet P-40, which led to total loss of activity. No bacteriocin adsorption was detected at pH 1 to 2, whereas 100% bacteriocin adsorption was found at pH 6.5. Based on Tricine SDS-PAGE the estimated molecular mass of bacteriocin was 54聽kDa. No plasmid was found to present in the isolate.