New Bacteriocin from Bacillus clausii StrainGM17: Purification, Characterization, and Biological Activity

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• 作者：Ghadbane Mouloud (1) (2) r> Harzallah Daoud (1) r> Jaouadi Bassem (3) r> Ibn Laribi Atef (4) r> Belhadj Hani (1) r>
• 关键词：Bacillus clausii ; Bacteriocin ; Purification ; Thermostability ; Bactericidal mode of action
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：171
• 期：8
• 页码：2186-2200
• 全文大小：
• 作者单位：Ghadbane Mouloud (1) (2) r> Harzallah Daoud (1) r> Jaouadi Bassem (3) r> Ibn Laribi Atef (4) r> Belhadj Hani (1) r>r>1. Laboratory of Applied Microbiology, Department of Microbiology, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif, 19000, Algeria r> 2. Laboratory of Plant Biotechnology, Department of Natural and Life Sciences, Faculty of Sciences, University of Mila, PO Box 166, Chebilia, Mila, 28000, Algeria r> 3. Centro de Tecnolog Poscosecha, Instituto Valenciano de Investigaciones Agrarias, 46113, Moncada, Valencia, Spain r> 4. Laboratory of Microorganisms and Biomolecules, Centre of Biotechnology of Sfax, University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax, 3018, Tunisia r>
• ISSN：1559-0291

文摘

A bacteriocin-producing strain (9,000AU/ml) was isolated from the rhizosphere of Algerian healthy plants Ononis angustissima Lam. and identified as Bacillus clausii strain GM17. The bacteriocin, called Bac-GM17, was purified from the culture supernatant after heat treatment, ammonium sulfate precipitation, Sephadex G-50 chromatography and Mono Q fast-performance liquid chromatography (FPLC). Based on matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis, the purified Bac-GM17 is a monomer protein with a molecular mass of 5,158.11Da. The N-terminal sequencing allowed for the straightforward identification of its first 20 residues, which were of pure bacteriocin. It also revealed that this bacteriocin contained a unique sequence, namely DWTCSKWSCLVCDDCSVELT, which suggests the identification of a novel compound. Bac-GM17 was extremely heat stable (20min at 120) and was stable within the pH range (3). It was found to be resistant to the proteolytic action of trypsin, pepsin, papain, pronase E, and proteinase K. It was also noted to display a bactericidal mode of action against Agrobacterium tumefaciens C58 and a fungistatic mode of action against Candida tropicalis R2 CIP203.