Bacteriocin production and safety evaluation of non-starter Enterococcus faecium IM1 and Enterococcus hirae IM1 strains isolated from homemade Egyptian dairy products

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• 作者：S. El-Ghaish ; A. El-Baz ; N. Hwanhlem ; M. Zommara…
• 关键词：Bacteriocin ; Lactic acid bacteria ; Enterococcus ; Egyptian dairy products
• 刊名：European Food Research and Technology
• 出版年：2015
• 出版时间：June 2015
• 年：2015
• 卷：240
• 期：6
• 页码：1211-1223
• 全文大小：1,078 KB
• 参考文献：1.El-Soda M, El-Ziney M, Awad S, Osman G, Omran N, Gamal G, Ezzat N, El-Shafei H (2003) A culture collection of lactic acid bacteria isolated from raw milk and traditional Egyptian dairy products. Egypt J Dairy Sci 31:23-1r>2.Ayad EHE, Nashat S, El-Sadek N, Metwaly H, El-Soda M (2004) Selection of wild lactic acid bacteria isolated from traditional Egyptian dairy products according to production and technological criteria. Food Micro 21:715-25View Article r>3.Sánchez-González L, Saavedra JIQ, Chiralt A (2014) Antilisterial and physical properties of biopolymer films containing lactic acid bacteria. Food Control 35:200-06View Article r>4.Klaenhammer TR (1993) Genetics of bacteriocins produced by lactic acid bacteria. FEMS Microbiol Rev 12:39-6View Article r>5.Foulquié Moreno MR, Sarantinopoulos P, Tsakalidou E, De Vuyst L (2006) The role and application of Enterococci in food and health. Int J Food Microbiol 106:1-4View Article r>6.Zouhir A, Hammami R, Fliss I, Ben Hamida J (2010) A new structure-based classification of Gram-positive bacteriocins. Protein J 29:432-39View Article r>7.Franz CM, Holzapfel WH, Stiles ME (1999) Enterococci at the crossroads of food safety. Int J Food Microbiol 47:1-4View Article r>8.Giraffa G (2003) Functionality of enterococci in dairy products. Int J Food Microbiol 88:215-22View Article r>9.Ayad EHE (2009) Starter culture development for improving safety and quality of Domiati cheese. Food Microbiol 26:533-41View Article r>10.El-Ghaish S, Hadji-Sfaxi I, Ahmadova A, Choiset Y, Rabesona H, Sitohy M, Haertlé T, Chobert J-M (2011) Characterization of two safe Enterococcus strains producing enterocins isolated from Egyptian dairy products. Benef Microbes 2:15-7View Article r>11.Hadji-Sfaxi I, El-Ghaish S, Ahmadova A, Batdorj B, Le Blay-Lalibré G, Barbier G, Haertlé T, Chobert J-M (2011) Antimicrobial activity and safety of use of Enterococcus faecium PC4.1. Food Control 22:2020-027View Article r>12.Centeno JA, Menendez S, Rodriguez-Otero JL (1996) Main microbial flora present as natural starters in Cebreiro raw cow’s milk cheese (Northwest Spain). Int J Food Microbiol 33:307-13View Article r>13.Litopoulou-Tzanetaki E, Tzanetakis N, Vafopoulou-Mastrojiannaki A (1993) Effect of type of lactic starter on microbiological, chemical and sensory characteristics of Feta cheese. Food Microbiol 10:31-1View Article r>14.Bover-Cid S, Hugas M, Izquierdo-Pulido M, Vidal-Carou MC (2001) Amino acid decarboxylase activity of bacteria isolated from fermented pork sausages. Int J Food Microbiol 66:185-89View Article r>15.Advisory Committee on Novel Foods and Processes (ACNFP) (1997) Report on Enterococcus faecium, strain K77D. Ergon House c/o Nobel House, 17 Smith square, London SW1 3JR, UKr>16.International Dairy Federation (IDF) (1997) Dairy starter cultures lactic acid bacteria (LAB), Standard 149A. International Dairy Federation, Brusselsr>17.Aymerich T, Holo H, Havarstein LS, Hugas M, Garriga M, Nes IF (1996) Biochemical and genetic characterization of enterocin A from Enterococcus faecium, a new antilisterial bacteriocin in the pediocin family of bacteriocins. Appl Environ Microbiol 62:1676-682 r>18.Aymerich T, Garriga M, Ylla J, Vallier J, Monfort JM, Hugas M (2000) Application of enterocins as biopreservatives against Listeria innocua in meat products. J Food Prot 63:721-26 r>19.Franz CM, van Belkum MJ, Holzapfel WH, Abriouel H, Gálvez A (2007) Diversity of enterococcal bacteriocins and their grouping in a new classification scheme. FEMS Microbiol Rev 31:293-10View Article r>20.Cintas LM, Casaus P, Havarstein LS, Hernandez PE, Nes IF (1997) Biochemical and genetic characterization of enterocin P, a novel sec-dependent bacteriocin from Enterococcus faecium P13 with a broad antimicrobial spectrum. Appl Environ Microbiol 63:4321-330 r>21.Ennahar S, Asou Y, Zendo T, Sonomoto K, Ishizaki A (2000) Biochemical and genetic evidence for production of enterocins A and B by Enterococcus faecium WHE 81. Int J Food Microbiol 70:291-01View Article r>22.van Belkum MJ, Hayema BJ, Jeeninga RA, Kok J, Venema G (1991) Organization and nucleotide sequences of two lactococcal bacteriocin operons. Appl Environ Microbiol 57:493-98r>23.Tahara T, Yoshioki S, Utsumi R, Kanatani K (1997) Isolation and partial characterization of bacteriocin produced by Lactobacillus gasseri JCM 2124. FEMS Microbiol Lett 148:97-00View Article r>24.Floriano B, Ruiz-Barba JL, Jiménez-Diaz R (1998) Purification and genetic characterization of enterocin I from Enterococcus faecium 6T1a, a novel antilisterial plasmid-encoded bacteriocin which does not belong to the pediocin family of bacteriocins. Appl Environ Microbiol 64:4883-890 r>25.Cintas LM, Casaus P, Herranz C, H?varstein LS, Holo H, Hernandez PE, Nes IF (2000) Biochemical and genetic evidence that Enterococcus faecium L50 produces enterocins L50A and L50B, the sec-dependent enterocin P, and a novel bacteriocin secre
• 作者单位：S. El-Ghaish (1) r> A. El-Baz (2) r> N. Hwanhlem (3) r> M. Zommara (1) r> E. Ayad (4) r> Y. Choiset (5) r> T. Haertlé (5) r> J.-M. Chobert (5) r>r>1. Department of Dairy Sciences, Faculty of Agriculture, Kafrelsheikh University, B.P. 33516, Kafr el-Sheikh, Egypt r> 2. Department of Dairy Science, Agriculture Research Center, Animal Production Research Institute, Sakha, Kafr el-Sheikh, 33717, Egypt r> 3. Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, 90112, Thailand r> 4. Department of Food Science, Faculty of Agriculture - Saba Basha, Alexandria University, P.O. Box 21531, Bolkely-Alexandria, Egypt r> 5. UR 1268 Biopolymères Interactions Assemblages, équipe Fonctions et Interactions des Protéines, INRA, 44316, Nantes Cedex 03, France r>
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistryr>Food Sciencer>Analytical Chemistryr>Biotechnologyr>Agriculturer>Forestryr>
• 出版者：Springer Berlin / Heidelberg
• ISSN：1438-2385

文摘

This study presents the characterization of some strains of lactic acid bacteria (LAB) from traditional Egyptian dairy products. Isolated LAB (623 isolates) were studied for their antimicrobial activity against taxonomically related microorganisms. Selected LAB were identified by PCR method as Enterococcus faecium IM1 and Enterococcus hirae IM1. Partially purified enterocins showed antimicrobial activity against Lactobacillus bulgaricus 340, Lb. brevis F104 and F145, Lb. sakei, Brochothrix thermosphacta DSMZ20171T and DSMZ20599, Carnobacterium maltaromaticum CIP103135T, C. piscicola S4312, E. faecalis JH2-2 and JH2-2rr04, E. faecium CTC492/t1362 and P13, Listeria grayi CLIP12518, L. innocua 1, CIP80.11T, F and P, L. ivanovii ATCC and CIP78.42T, L. monocytogenes CIP78.35, DSM12464, EGDe, RF124, RF125, RF131, RF132, RF133, RF142, RF151 and RF152, and Vagococcus penaei CIP 109914T but have no effect against Bacillus subtilis 168T, Moellerella wisconsensis MIP2451, Morganella psychrotolerans MIP2488, Pseudomonas fluorescens 10, Salmonella enterica, Sal. montevideo, Sal. typhimurium and Serratia liquefaciens CIP103238T. The inhibitory activity was not due to hydrogen peroxide for E. hirae IM1, but strain E. faecium IM1 may excrete diverse antimicrobial compounds such as hydrogen peroxide and bacteriocins. Antimicrobial activity of E. faecium IM1 was initially detected during exponential phase of growth, and the maximal level (1,300?AU/mL) was observed at 12?h and remained stable till the end of incubation time (48?h). Maximum of activity of E. hirae IM1 was observed during the logarithmic phase (6?h) and then decreased after 12?h. Bacteriocins produced by E. faecium IM1 and E. hirae IM1 were stable between pH 5 and pH 8 and stable also until 100?°C/20?min. Tested strains were free from virulence determinant genes as well as hemolytic and gelatinase activities. E. faecium IM1 was sensitive to penicillin, kanamycin, vancomycin, gentamicin and tetracycline but resistant to ampicillin. Otherwise, E. hirae IM1 was sensitive to penicillin, kanamycin, vancomycin and tetracycline but resistant to ampicillin and gentamicin. The isolated cultures with antimicrobial activities may be used as safe and useful starter cultures or co-cultures.