DNA methylation differences between in vitro- and in vivo-conceived children are associated with ART procedures rather than infertility

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• 作者：Sisi Song ; Jayashri Ghosh ; Monica Mainigi ; Nahid Turan…
• 关键词：DNA methylation ; Assisted reproduction ; Donor oocytes ; Infertility ; Placenta
• 刊名：Clinical Epigenetics
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：7
• 期：1
• 全文大小：770 KB
• 刊物主题：Human Genetics; Gene Function;
• 出版者：BioMed Central
• ISSN：1868-7083

文摘

Background We, and others, have demonstrated previously that there are differences in DNA methylation and transcript levels of a number of genes in cord blood and placenta between children conceived using assisted reproductive technologies (ART) and children conceived in vivo. The source of these differences (the effect of ART versus the underlying infertility) has never been determined in humans. In this study, we have attempted to resolve this issue by comparing placental DNA methylation levels at 37 CpG sites in 16 previously identified candidate genes in independent populations of children conceived in vivo (‘fertile control’ group) with ART children conceived from two groups: either autologous oocytes with infertility in one or both parents (‘infertile ART’ group) or donor oocytes (obtained from young fertile donors) without male infertility (‘donor oocyte ART’ group).