Enzymes of sucrose dissimilation as targets for nitrate in early ontogenesis of garden pea

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• 作者：A. V. Nikitin ; S. F. Izmailov
• 关键词：Pisum sativum ; sucrose synthase ; acid and alkaline invertases ; nitrate ; nitrate signaling ; heterotrophy ; autotrophy
• 刊名：Russian Journal of Plant Physiology
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：63
• 期：1
• 页码：152-157
• 全文大小：280 KB
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• 作者单位：A. V. Nikitin (1)
  S. F. Izmailov (1)
  
  1. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, ul. Botanicheskaya 35, Moscow, 127276, Russia
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Physiology
  Plant Sciences
  Russian Library of Science
  
• 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
• ISSN：1608-3407

文摘

We experimentally tested a possibility that nitrate regulates sucrose synthase (SS) in the roots, vacuolar acid invertase (AcI) in the stems, and alkaline invertase (AlI) in the leaves of garden pea (Pisum sativum L.) where they were predominantly located. In the course of early ontogenesis of the seedlings (0–20 days), NO ₃ - stimulated solely SS. We detected three peaks of nitrate-dependent enzyme activity: the greatest peak was observed in the very beginning of germination (48 h from seed imbibition), the intermediate peak corresponded to the most intense utilization of cotyledonal reserves (sixth to eighth day of growth), and the lowest peak coincided with development of autotrophy (tenth to 20th day). In the period of their greatest activity (sixth to 14th day), neither invertases noticeably respond to nitrate. At the first peak, NO ₃ - affected SS already at a concentration of 1 mM; this influence was maintained at concentrations rising to 10 mM, which points to a signal nature of the effect. We discuss a functional aspect of the effect of nitrate on SS involved in the system of the enzymes of sucrose dissimilation upon the initiation and development of different physiological processes occurring in this period, as well as its possible applied role in agricultural biotechnologies.