Silver nanoparticle induced cytotoxicity, oxidative stress, and DNA damage in CHO cells

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• 作者：Kumud Kant Awasthi (1)
  Anjali Awasthi (2)
  Narender Kumar (2)
  Partha Roy (2)
  Kamlendra Awasthi (3)
  P. J. John (1)
  
• 关键词：Ag nanoparticles ; UV–Vis spectroscopy ; TEM ; Cytotoxicity ; DNA damage
• 刊名：Journal of Nanoparticle Research
• 出版年：2013
• 出版时间：September 2013
• 年：2013
• 卷：15
• 期：9
• 全文大小：706KB
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• 作者单位：Kumud Kant Awasthi (1)
  Anjali Awasthi (2)
  Narender Kumar (2)
  Partha Roy (2)
  Kamlendra Awasthi (3)
  P. J. John (1)
  
  1. Department of Zoology, University of Rajasthan, Jaipur, 302004, India
  2. Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, 247667, India
  3. Department of Physics, Malaviya National Institute of Technology, Jaipur, 302017, India
  

文摘

Silver nanoparticles (Ag NPs) are being used increasingly in wound dressings, catheters, and in various household products due to their antimicrobial activity. The present study reports the toxicity evaluation of synthesized and well characterized Ag NPs using Chinese hamster ovary (CHO) cells. The UV–Vis spectroscopy reveals the formation of silver nanoparticles by exhibiting the typical surface plasmon absorption maxima at 408-10?nm. Transmission electron microscopy (TEM) reveals that the average diameter of silver nanoparticles is about 5.0?±?1.0?nm and that they have spherical shape. Cell visibility and cell viability percentage show dose-dependent cellular toxicity of Ag NPs. The half maximal inhibitory concentration (IC50) for CHO cells is 68.0?±?2.65?μg/ml after 24?h Ag NPs exposure. Toxicity evaluations, including cellular morphology, mitochondrial function (MTT assay), reactive oxygen species (ROS), and DNA fragmentation assay (Ladder pattern) were assessed in unexposed CHO cells (control) and the cells exposed to Ag NPs concentrations of 15, 30, and 60?μg/ml for 24?h. The findings may assist in the designing of Ag NPs for various applications and provide insights into their toxicity.