Production of Limonoids with Insect Antifeedant Activity in a Two-Stage Bioreactor Process with Cell Suspension Culture of Azadirachta indica

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• 作者：Andrés Vásquez-Rivera ; Diego Chicaiza-Finley…
• 关键词：Azadirachta indica ; Plant cell culture ; Bioreactor ; Two ; stage cultivation ; Limonoids ; Antifeedant activity
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：177
• 期：2
• 页码：334-345
• 全文大小：1,058 KB
• 参考文献：1.Aktar, M. W., Sengupta, D., & Chowdhury, A. (2009). Impact of pesticides use in agriculture: their benefits and hazards. Interdisciplinary Toxicology, 2(1), 1-2. doi:10.-478/?v10102-009-0001-7 .CrossRef
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  4.Prakash, G., Bhojwani, S. S., & Srivastava, A. K. (2002). Production of azadirachtin from plant tissue culture: state of the art and future prospects. Biotechnology and Bioprocess Engineering, 7(4), 185-93. doi:10.-007/?BF02932968 .CrossRef
  5.Srivastava, S., & Srivastava, A. K. (2013). Production of the biopesticide azadirachtin by hairy root cultivation of Azadirachta indica in liquid-phase bioreactors. Applied Biochemistry and Biotechnology, 171(6), 1351-361.CrossRef
  6.Srivastava, S., & Srivastava, A. K. (2014). Effect of elicitors and precursors on azadirachtin production in hairy root culture of Azadirachta indica. Applied Biochemistry and Biotechnology, 172(4), 2286-297. doi:10.-007/?s12010-013-0664-6 .CrossRef
  7.Prakash, G., & Srivastava, A. K. (2008). Production of biopesticides in an in situ cell retention bioreactor. Applied Biochemistry and Biotechnology, 151(2-3), 307-18. doi:10.-007/?s12010-008-8191-6 .CrossRef
  8.Srivastava, S., & Srivastava, A. K. (2012). Azadirachtin production by hairy root cultivation of Azadirachta indica in a modified stirred tank reactor. Bioprocess and Biosystems Engineering, 35(9), 1549-553. doi:10.-007/?s00449-012-0745-x .CrossRef
  9.Srivastava, S., & Srivastava, A. K. (2012). In vitro azadirachtin production by hairy root cultivation of Azadirachta indica in nutrient mist bioreactor. Applied Biochemistry and Biotechnology, 166(2), 365-78. doi:10.-007/?s12010-011-9430-9 .CrossRef
  10.Srivastava, S., & Srivastava, A. K. (2012). Strategies to overcome oxygen transfer limitations during hairy root cultivation of Azadiracta indica for enhanced azadirachtin production. Applied Biochemistry and Biotechnology, 167(6), 1818-830. doi:10.-007/?s12010-011-9531-5 .CrossRef
  11.Prakash, G., & Srivastava, A. K. (2007). Azadirachtin production in stirred tank reactors by Azadirachta indica suspension culture. Process Biochemistry, 42(1), 93-7. doi:10.-016/?j.?procbio.-006.-6.-20 .CrossRef
  12.Singh, M., & Chaturvedi, R. (2013). Sustainable production of azadirachtin from differentiated in vitro cell lines of neem (Azadirachta indica A. Juss.). AoB Plants, 5, plt034–plt034. doi:10.-093/?aobpla/?plt034 .
  13.Simmonds, M. S. J., Jarvis, A. P., Johnson, S., Jones, G. R., & Morgan, E. D. (2004). Comparison of anti-feedant and insecticidal activity of nimbin and salannin photo-oxidation products with neem (Azadirachta indica) limonoids. Pest Management Science, 60(5), 459-64.CrossRef
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  16.Capataz-Tafur, J., Orozco-Sánchez, F., Vergara-Ruiz, R., & Hoyos-Sánchez, R. (2007). Antifeedant effect of cell suspension extracts of Azadirachta indica on Spodoptera frugiperda J. E. Smith under laboratory conditions. Revista Facultad Nacional de Agronomía, Medellín, 60(1), 3703-715. Retrieved from http://?www.?scielo.?org.?co/?scielo.?php??script=?sci_?arttext&?pid=?S0304-2847200700010000-&?lng=?en&?nrm=?iso&?tlng=?es
  17.Orozco-Sánchez, F. (2009). Efecto de la oferta oxígeno sobre el crecimiento y la producción de terpenoides con células de Azadirachta indica en un biorreactor. Yautepec, México, PhD thesis Instituto Politécnico Nacional. Retrieved from http://?itzamna.?bnct.?ipn.?mx:-080/?dspace/?bitstream/-23456789/-725/-/?EFECTOFERTA.?pdf .
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  20.Prakash, G., & Srivastava, A. K. (2008). Statistical elicitor optimization studies for the enhance
• 作者单位：Andrés Vásquez-Rivera (1)
  Diego Chicaiza-Finley (1)
  Rodrigo A. Hoyos (1)
  Fernando Orozco-Sánchez (1)
  
  1. Grupos de Biotecnología Industrial y Biotecnología Vegetal, Facultad de Ciencias, Universidad Nacional de Colombia Sede Medellín, Calle 59A No 63- 20, Medellín, Colombia
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Biochemistry
  
• 出版者：Humana Press Inc.
• ISSN：1559-0291

文摘

Neem tree (Azadirachta indica) cell suspension culture is an alternative for the production of limonoids for insect control that overcomes limitations related to the supply of neem seeds. To establish conditions for cell growth and azadiracthin-related limonoid production, the effect of different sucrose concentrations, nitrate and phosphate in Murashige and Skoog (MS) medium, and the addition of one precursor and three elicitors was evaluated in shake flasks. The process was scaled up to a 3-l stirred tank bioreactor in one- and two-stage batch cultivation. In shake flasks, more than fivefold increase in the production of limonoids with the modified MS medium was observed (increase from 0.77 to 4.52 mg limonoids/g dry cell weight, DCW), while an increase of more than fourfold was achieved by adding the elicitors chitosan, salicylic acid, and jasmonic acid together (increase from 1.03 to 4.32 mg limonoids/g DCW). In the bioreactor, the volumetric production of limonoids was increased more than threefold with a two-stage culture in day 18 (13.82 mg limonoids/l in control single-stage process and 41.44 mg/l in two-stage process). The cultivation and operating mode of the bioreactor reported in this study may be adapted and used in optimization and process plant development for production of insect antifeedant limonoids with A. indica cell suspension cultures. Keywords Azadirachta indica Plant cell culture Bioreactor Two-stage cultivation Limonoids Antifeedant activity