Antioxidant Levels and Activities of Reactive Oxygen-Scavenging Enzymes in Crab Apple Fruits (ss="a-plus-plus">Malus baccata)

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• 作者：Ruchika Sharma ; Amarjit K Nath
• 关键词：Antioxidant activity ; Crab apple ; Purification ; Superoxide dismutase
• 刊名：Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：86
• 期：4
• 页码：877-885
• 全文大小：487 KB
• 刊物主题：Life Sciences, general; Behavioural Sciences; Plant Biochemistry; Nucleic Acid Chemistry;
• 出版者：Springer India
• ISSN：2250-1746
• 卷排序：86

文摘

Crab apple (Malus baccata) fruits show higher antioxidant activity, phenols and activities of antioxidant enzymes namely, ascorbate oxidase, catalase, peroxidase, superoxide dismutase except ascorbic acid content as compared to ‘Red Delicious’. Superoxide dismutase was purified and characterized from pulp extract of crab apple fruits by ammonium sulphate precipitation, gel filtration and ion exchange chromatography. Three isoforms of superoxide dismutase were eluted with water, 0.1 molar (M) and 0.2 M potassium chloride (KCl) on anion exchanger. The molecular weight of purified superoxide dismutase isoforms were 15, 17 and 20 kilodaltons (kDa), respectively. Superoxide dismutase isoforms were heat stable up to 90 °C and had pH optima of 7.8. The activity of all the three isoforms increased with increase in concentrations of zinc (Zn2+) ions in the reaction mixture. Keeping in view the importance of superoxide dismutase enzyme as a free radical scavenger and its role in stress tolerance in plants, there is a need to identify novel genes of this enzyme from native germplasm.