Characterization of a New 1,3-1,4-β-Glucanase Gene from Bacillus tequilensis CGX5-1
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- 作者：Jinjing Wang (1)
Chengtuo Niu (1)
Xiaoling Liu (1)
Xi Chen (1)
Qi Li (1) - 关键词：Bacillus tequilensis CGX5 ; 1 ; 1 ; 3 ; 1 ; 4 ; β ; Glucanase ; bgl5 ; 1 ; Brewing
- 刊名：Applied Biochemistry and Biotechnology
- 出版年：2014
- 出版时间：June 2014
- 年：2014
- 卷：173
- 期：3
- 页码：826-837
- 全文大小：
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Chengtuo Niu (1)
Xiaoling Liu (1)
Xi Chen (1)
Qi Li (1)
1. Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, Jiangsu, China - ISSN：1559-0291
文摘
1,3-1,4-β-Glucanase received great interest due to its application in brewing and feed industries. Application of 1,3-1,4-β-glucanase in brewing industry helps make up for the defect that plant-derived β-glucanases are heat-sensitive. A new strain, CGX5-1, exhibited remarkable 1,3-1,4-β-glucanase, was isolated from Asian giant hornet nest and identified Bacillus tequilensis. Moreover, a new 1,3-1,4-β-glucanase gene from B. tequilensis was cloned and measured to be 720?bp encoding 239 amino acids, with a predicted molecular weight of 26.9?kDa. After expressed in Escherichia coli BL21, active recombinant enzyme of 24?kDa was detected in the supernatant of cell culture, with the activity of 2,978.2 U/mL. The new enzyme was stable in the pH?5.0-.5 with the highest activity measured at pH?6.0. Moreover, it is thermostable within 45 to 60?°C. The property of the new recombinant enzyme makes this enzyme a broad prospect in brewing industry. Moreover, this is the first report on 1,3-1,4-β-glucanase produced by B. tequilensis.